Expression of bacterial genes and induction of INF-γ in human myeloid dendritic cells during persistent infection with Chlamydophila pneumoniae

Zoltan Kis, Balint Treso, K. Burián, V. Endrész, E. Pállinger, Agnes Nagy, A. Tóth, M. Takács, A. Falus, E. Gönczöl

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Abstract

The interactions between human monocyte-derived dendritic cells (DCs) and Chlamydophila pneumoniae (Cpn) infection were investigated. Cpn infection induced the maturation and functional activation of DCs, and Cpn antigens were present in all of the subpopulations during the maturation process. Chlamydial antigens were detected in DCs during an observation period of 28 days. The exponential production of infectious elementary bodies was not observed. Chlamydial transcripts of the 16S rRNA gene, groEL-1 and omcB genes were expressed, as determined by a quantitative real-time PCR, but the expression of the ftsK gene was limited. DC cultures produced IFN-γ, but the presence of IFN-γ in the culture medium was not the major factor that decreased the growth of Cpn, as was shown by neutralization of the IFN-γ. A cell population identified as producing IFN-γ had no markers for T, B, natural killer, monocyte cells or macrophages but displayed DC morphology and the expression of specific DC markers, such as CD11c and HLA-DR. These results reveal a persistent infection of DCs with the expression of some, but not cell division-related genes and the production of IFN-γ that may contribute to the pathomechanism of chronic inflammatory diseases associated with persistent Cpn infection.

Original languageEnglish
Pages (from-to)324-334
Number of pages11
JournalFEMS Immunology and Medical Microbiology
Volume52
Issue number3
DOIs
Publication statusPublished - Apr 2008

Fingerprint

Bacterial Genes
Chlamydophila pneumoniae
Myeloid Cells
Dendritic Cells
Infection
Monocytes
Antigens
HLA-DR Antigens
rRNA Genes
Natural Killer Cells
Cell Division
Genes
Culture Media
Real-Time Polymerase Chain Reaction
Intercellular Signaling Peptides and Proteins
Chronic Disease
Cell Culture Techniques
Macrophages
Observation
Gene Expression

Keywords

  • Chlamydophila pneumoniae
  • Dendritic cells
  • IFN-γ
  • Infection
  • mRNA expression

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Infectious Diseases

Cite this

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title = "Expression of bacterial genes and induction of INF-γ in human myeloid dendritic cells during persistent infection with Chlamydophila pneumoniae",
abstract = "The interactions between human monocyte-derived dendritic cells (DCs) and Chlamydophila pneumoniae (Cpn) infection were investigated. Cpn infection induced the maturation and functional activation of DCs, and Cpn antigens were present in all of the subpopulations during the maturation process. Chlamydial antigens were detected in DCs during an observation period of 28 days. The exponential production of infectious elementary bodies was not observed. Chlamydial transcripts of the 16S rRNA gene, groEL-1 and omcB genes were expressed, as determined by a quantitative real-time PCR, but the expression of the ftsK gene was limited. DC cultures produced IFN-γ, but the presence of IFN-γ in the culture medium was not the major factor that decreased the growth of Cpn, as was shown by neutralization of the IFN-γ. A cell population identified as producing IFN-γ had no markers for T, B, natural killer, monocyte cells or macrophages but displayed DC morphology and the expression of specific DC markers, such as CD11c and HLA-DR. These results reveal a persistent infection of DCs with the expression of some, but not cell division-related genes and the production of IFN-γ that may contribute to the pathomechanism of chronic inflammatory diseases associated with persistent Cpn infection.",
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author = "Zoltan Kis and Balint Treso and K. Buri{\'a}n and V. Endr{\'e}sz and E. P{\'a}llinger and Agnes Nagy and A. T{\'o}th and M. Tak{\'a}cs and A. Falus and E. G{\"o}ncz{\"o}l",
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T1 - Expression of bacterial genes and induction of INF-γ in human myeloid dendritic cells during persistent infection with Chlamydophila pneumoniae

AU - Kis, Zoltan

AU - Treso, Balint

AU - Burián, K.

AU - Endrész, V.

AU - Pállinger, E.

AU - Nagy, Agnes

AU - Tóth, A.

AU - Takács, M.

AU - Falus, A.

AU - Gönczöl, E.

PY - 2008/4

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N2 - The interactions between human monocyte-derived dendritic cells (DCs) and Chlamydophila pneumoniae (Cpn) infection were investigated. Cpn infection induced the maturation and functional activation of DCs, and Cpn antigens were present in all of the subpopulations during the maturation process. Chlamydial antigens were detected in DCs during an observation period of 28 days. The exponential production of infectious elementary bodies was not observed. Chlamydial transcripts of the 16S rRNA gene, groEL-1 and omcB genes were expressed, as determined by a quantitative real-time PCR, but the expression of the ftsK gene was limited. DC cultures produced IFN-γ, but the presence of IFN-γ in the culture medium was not the major factor that decreased the growth of Cpn, as was shown by neutralization of the IFN-γ. A cell population identified as producing IFN-γ had no markers for T, B, natural killer, monocyte cells or macrophages but displayed DC morphology and the expression of specific DC markers, such as CD11c and HLA-DR. These results reveal a persistent infection of DCs with the expression of some, but not cell division-related genes and the production of IFN-γ that may contribute to the pathomechanism of chronic inflammatory diseases associated with persistent Cpn infection.

AB - The interactions between human monocyte-derived dendritic cells (DCs) and Chlamydophila pneumoniae (Cpn) infection were investigated. Cpn infection induced the maturation and functional activation of DCs, and Cpn antigens were present in all of the subpopulations during the maturation process. Chlamydial antigens were detected in DCs during an observation period of 28 days. The exponential production of infectious elementary bodies was not observed. Chlamydial transcripts of the 16S rRNA gene, groEL-1 and omcB genes were expressed, as determined by a quantitative real-time PCR, but the expression of the ftsK gene was limited. DC cultures produced IFN-γ, but the presence of IFN-γ in the culture medium was not the major factor that decreased the growth of Cpn, as was shown by neutralization of the IFN-γ. A cell population identified as producing IFN-γ had no markers for T, B, natural killer, monocyte cells or macrophages but displayed DC morphology and the expression of specific DC markers, such as CD11c and HLA-DR. These results reveal a persistent infection of DCs with the expression of some, but not cell division-related genes and the production of IFN-γ that may contribute to the pathomechanism of chronic inflammatory diseases associated with persistent Cpn infection.

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