Expression of active human blood clotting factor VIII in the mammary gland of transgenic rabbits

L. Hiripi, F. Makovics, R. Halter, M. Baranyi, D. Paul, J. W. Carnwath, Z. Bösze, H. Niemann

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Human clotting factor VIII is probably the largest protein to be expressed to date in the mammary gland of a transgenic animal, and it requires extensive posttranslational modification to achieve full biological activity. The mammary gland specific construct mWAP-hFVIII-MT-I was injected into the pronuclei of rabbit zygotes, and three transgenic offspring were obtained. Founder 385 showed germ-line transmission of a single integrated copy, and a homozygous line was established from this animal. The rhFVIII was transcribed and translated exclusively in the mammary gland. The activity of rhFVIII in the rabbit milk ranged from 5 to 8% of that found in normal human plasma. Results indicate the suitability of the transgenic rabbit mammary gland for rhFVIII production.

Original languageEnglish
Pages (from-to)41-45
Number of pages5
JournalDNA and Cell Biology
Volume22
Issue number1
DOIs
Publication statusPublished - Jan 1 2003

Fingerprint

Blood Coagulation Factors
Factor VIII
Blood Coagulation
Human Mammary Glands
Rabbits
Genetically Modified Animals
Zygote
Post Translational Protein Processing
Germ Cells
Milk
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Hiripi, L., Makovics, F., Halter, R., Baranyi, M., Paul, D., Carnwath, J. W., ... Niemann, H. (2003). Expression of active human blood clotting factor VIII in the mammary gland of transgenic rabbits. DNA and Cell Biology, 22(1), 41-45. https://doi.org/10.1089/104454903321112488

Expression of active human blood clotting factor VIII in the mammary gland of transgenic rabbits. / Hiripi, L.; Makovics, F.; Halter, R.; Baranyi, M.; Paul, D.; Carnwath, J. W.; Bösze, Z.; Niemann, H.

In: DNA and Cell Biology, Vol. 22, No. 1, 01.01.2003, p. 41-45.

Research output: Contribution to journalArticle

Hiripi, L, Makovics, F, Halter, R, Baranyi, M, Paul, D, Carnwath, JW, Bösze, Z & Niemann, H 2003, 'Expression of active human blood clotting factor VIII in the mammary gland of transgenic rabbits', DNA and Cell Biology, vol. 22, no. 1, pp. 41-45. https://doi.org/10.1089/104454903321112488
Hiripi, L. ; Makovics, F. ; Halter, R. ; Baranyi, M. ; Paul, D. ; Carnwath, J. W. ; Bösze, Z. ; Niemann, H. / Expression of active human blood clotting factor VIII in the mammary gland of transgenic rabbits. In: DNA and Cell Biology. 2003 ; Vol. 22, No. 1. pp. 41-45.
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