Expression of Active Fluorophore Proteins in the Milk of Transgenic Pigs Bypassing the Secretory Pathway

Ayan Mukherjee, Wiebke Garrels, Thirumala R. Talluri, Daniela Tiedemann, Zsuzsanna Bosze, Zoltán Ivics, Wilfried A. Kues

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We describe the expression of recombinant fluorescent proteins in the milk of two lines of transgenic pigs generated by Sleeping Beauty transposon-mediated genetic engineering. The Sleeping Beauty transposon consisted of an ubiquitously active CAGGS promoter driving a fluorophore cDNA, encoding either Venus or mCherry. Importantly, the fluorophore cDNAs did not encode for a signal peptide for the secretory pathway, and in previous studies of the transgenic animals a cytoplasmic localization of the fluorophore proteins was found. Unexpectedly, milk samples from lactating sows contained high levels of bioactive Venus or mCherry fluorophores. A detailed analysis suggested that exfoliated cells of the mammary epithelium carried the recombinant proteins passively into the milk. This is the first description of reporter fluorophore expression in the milk of livestock, and the findings may contribute to the development of an alternative concept for the production of bioactive recombinant proteins in the udder.

Original languageEnglish
Article number24464
JournalScientific reports
Volume6
DOIs
Publication statusPublished - Apr 18 2016

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Expression of Active Fluorophore Proteins in the Milk of Transgenic Pigs Bypassing the Secretory Pathway'. Together they form a unique fingerprint.

  • Cite this