Expression of a novel-type small proline-rich protein gene of alfalfa is induced by 2,4-dichlorophenoxiacetic acid in dedifferentiated callus cells

J. Györgyey, Kinga Németh, Z. Magyar, Zsolt Kelemen, Thierry Alliotte, Dirk Inzé, D. Dudits

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Differential screening of a cDNA library of 2,4-dichlorophenoxiacetic acid (2,4-D)-treated alfalfa (Medicago sativa) callus tissues resulted in the isolation of a 571 bp cDNA clone (MsPRP5) encoding for a proline-rich protein (84 amino acids) with a specific repeat unit of TPVLPPR(κ)/(R)GRPPPVPP. In addition, a characteristic amino acid block (PPVYK) previously found in other proline-rich proteins also occurs in the C-terminal region of MsPRP5. At the N-terminal, a signal peptide similar to leader sequences of extracellular proteins can be predicted. According to the northern analysis, the corresponding gene is not expressed or is weakly expressed in differentiated vegetative organs and somatic embryos. However the accumulation of MsPRP5 mRNA is auxin concentration-dependent in dedifferentiated callus tissue. An increase in the amount of steady-state mRNA was detected already 20 min after auxin shock (100 μM 2.4-D). Maximum expression was observed at 24-48 h in the presence of 2,4-D. Elevated expression was also found in cells recovering after heat shock and wounding stress. In synchronized alfalfa cells, the transcript level of MsPRP5 gene fluctuated during cell cycle progression with peaks in G1/S phase cells. Considering the structural features and expression properties of MsPRP5, this clone may represents a new type of proline-rich protein gene which responds to hormonal shock and some other stresses as well.

Original languageEnglish
Pages (from-to)593-602
Number of pages10
JournalPlant Molecular Biology
Volume34
Issue number4
DOIs
Publication statusPublished - Jul 1997

Fingerprint

Medicago sativa
Bony Callus
Proline
proline
alfalfa
callus
Shock
2,4-Dichlorophenoxyacetic Acid
Acids
Indoleacetic Acids
acids
2,4-D
auxins
Proteins
genes
proteins
Clone Cells
Genes
cells
Tissue

Keywords

  • Auxin response
  • cDNA
  • Cell cycle
  • Medicago sativa
  • Proline-rich protein
  • Stress response

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Expression of a novel-type small proline-rich protein gene of alfalfa is induced by 2,4-dichlorophenoxiacetic acid in dedifferentiated callus cells. / Györgyey, J.; Németh, Kinga; Magyar, Z.; Kelemen, Zsolt; Alliotte, Thierry; Inzé, Dirk; Dudits, D.

In: Plant Molecular Biology, Vol. 34, No. 4, 07.1997, p. 593-602.

Research output: Contribution to journalArticle

@article{99b71f5edcf74d4480429276297b7858,
title = "Expression of a novel-type small proline-rich protein gene of alfalfa is induced by 2,4-dichlorophenoxiacetic acid in dedifferentiated callus cells",
abstract = "Differential screening of a cDNA library of 2,4-dichlorophenoxiacetic acid (2,4-D)-treated alfalfa (Medicago sativa) callus tissues resulted in the isolation of a 571 bp cDNA clone (MsPRP5) encoding for a proline-rich protein (84 amino acids) with a specific repeat unit of TPVLPPR(κ)/(R)GRPPPVPP. In addition, a characteristic amino acid block (PPVYK) previously found in other proline-rich proteins also occurs in the C-terminal region of MsPRP5. At the N-terminal, a signal peptide similar to leader sequences of extracellular proteins can be predicted. According to the northern analysis, the corresponding gene is not expressed or is weakly expressed in differentiated vegetative organs and somatic embryos. However the accumulation of MsPRP5 mRNA is auxin concentration-dependent in dedifferentiated callus tissue. An increase in the amount of steady-state mRNA was detected already 20 min after auxin shock (100 μM 2.4-D). Maximum expression was observed at 24-48 h in the presence of 2,4-D. Elevated expression was also found in cells recovering after heat shock and wounding stress. In synchronized alfalfa cells, the transcript level of MsPRP5 gene fluctuated during cell cycle progression with peaks in G1/S phase cells. Considering the structural features and expression properties of MsPRP5, this clone may represents a new type of proline-rich protein gene which responds to hormonal shock and some other stresses as well.",
keywords = "Auxin response, cDNA, Cell cycle, Medicago sativa, Proline-rich protein, Stress response",
author = "J. Gy{\"o}rgyey and Kinga N{\'e}meth and Z. Magyar and Zsolt Kelemen and Thierry Alliotte and Dirk Inz{\'e} and D. Dudits",
year = "1997",
month = "7",
doi = "10.1023/A:1005845412667",
language = "English",
volume = "34",
pages = "593--602",
journal = "Plant Molecular Biology",
issn = "0167-4412",
publisher = "Springer Netherlands",
number = "4",

}

TY - JOUR

T1 - Expression of a novel-type small proline-rich protein gene of alfalfa is induced by 2,4-dichlorophenoxiacetic acid in dedifferentiated callus cells

AU - Györgyey, J.

AU - Németh, Kinga

AU - Magyar, Z.

AU - Kelemen, Zsolt

AU - Alliotte, Thierry

AU - Inzé, Dirk

AU - Dudits, D.

PY - 1997/7

Y1 - 1997/7

N2 - Differential screening of a cDNA library of 2,4-dichlorophenoxiacetic acid (2,4-D)-treated alfalfa (Medicago sativa) callus tissues resulted in the isolation of a 571 bp cDNA clone (MsPRP5) encoding for a proline-rich protein (84 amino acids) with a specific repeat unit of TPVLPPR(κ)/(R)GRPPPVPP. In addition, a characteristic amino acid block (PPVYK) previously found in other proline-rich proteins also occurs in the C-terminal region of MsPRP5. At the N-terminal, a signal peptide similar to leader sequences of extracellular proteins can be predicted. According to the northern analysis, the corresponding gene is not expressed or is weakly expressed in differentiated vegetative organs and somatic embryos. However the accumulation of MsPRP5 mRNA is auxin concentration-dependent in dedifferentiated callus tissue. An increase in the amount of steady-state mRNA was detected already 20 min after auxin shock (100 μM 2.4-D). Maximum expression was observed at 24-48 h in the presence of 2,4-D. Elevated expression was also found in cells recovering after heat shock and wounding stress. In synchronized alfalfa cells, the transcript level of MsPRP5 gene fluctuated during cell cycle progression with peaks in G1/S phase cells. Considering the structural features and expression properties of MsPRP5, this clone may represents a new type of proline-rich protein gene which responds to hormonal shock and some other stresses as well.

AB - Differential screening of a cDNA library of 2,4-dichlorophenoxiacetic acid (2,4-D)-treated alfalfa (Medicago sativa) callus tissues resulted in the isolation of a 571 bp cDNA clone (MsPRP5) encoding for a proline-rich protein (84 amino acids) with a specific repeat unit of TPVLPPR(κ)/(R)GRPPPVPP. In addition, a characteristic amino acid block (PPVYK) previously found in other proline-rich proteins also occurs in the C-terminal region of MsPRP5. At the N-terminal, a signal peptide similar to leader sequences of extracellular proteins can be predicted. According to the northern analysis, the corresponding gene is not expressed or is weakly expressed in differentiated vegetative organs and somatic embryos. However the accumulation of MsPRP5 mRNA is auxin concentration-dependent in dedifferentiated callus tissue. An increase in the amount of steady-state mRNA was detected already 20 min after auxin shock (100 μM 2.4-D). Maximum expression was observed at 24-48 h in the presence of 2,4-D. Elevated expression was also found in cells recovering after heat shock and wounding stress. In synchronized alfalfa cells, the transcript level of MsPRP5 gene fluctuated during cell cycle progression with peaks in G1/S phase cells. Considering the structural features and expression properties of MsPRP5, this clone may represents a new type of proline-rich protein gene which responds to hormonal shock and some other stresses as well.

KW - Auxin response

KW - cDNA

KW - Cell cycle

KW - Medicago sativa

KW - Proline-rich protein

KW - Stress response

UR - http://www.scopus.com/inward/record.url?scp=0031193913&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031193913&partnerID=8YFLogxK

U2 - 10.1023/A:1005845412667

DO - 10.1023/A:1005845412667

M3 - Article

C2 - 9247541

AN - SCOPUS:0031193913

VL - 34

SP - 593

EP - 602

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 0167-4412

IS - 4

ER -