Expression of βig-h3 is lower than normal in keratoconus corneas but increases with scarring

Lili Takács, Adrienne Csutak, Erzsébet Balázs, L. Módis, A. Berta

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Purpose. Keratoconus is a progressive ectatic disease of the cornea. Despite extensive clinical and laboratory investigations, its pathogenesis remains unclear. In this study, we examined the localization of βig-h3, a recently described extracellular matrix protein in keratoconus corneas both in the absence and presence of subepithelial scarring. Methods. Two normal corneas and central corneal buttons of 10 patients with keratoconus were excised during perforating keratoplasty and examined, including one case with acute corneal hydrops. In one case, keratoconus was associated with Down syndrome. Immunodetection was done with an antipeptide antibody reacting with the N-terminal part of βig-h3. Results. We found decreased βig-h3 levels in the basal epithelial layer and keratocytes of keratoconus corneas. In the scarred corneas, however, βig-h3 levels were increased in the basal epithelial layers and in activated keratocytes at the places of scarring. In the cornea of the patient with Down syndrome, we found an additional βig-h3- positive zone in the anterior stroma. Conclusions. The decreased levels of βig-h3 corneas seem to be specific for keratoconus. Considering the putative role of βig-h3 as a cellular-attachment protein, paucity of βig-h3 in the corneal stroma may lead to decreased mechanical stability and contribute to the development of keratoconus.

Original languageEnglish
Pages (from-to)599-605
Number of pages7
JournalCornea
Volume18
Issue number5
Publication statusPublished - Sep 1999

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Keratoconus
Cornea
Cicatrix
Down Syndrome
Corneal Stroma
Corneal Transplantation
Extracellular Matrix Proteins
Edema
Antibodies

Keywords

  • βig-h3
  • Extracellular matrix
  • Immunohistochemistry
  • Keratoconus
  • Pathogenesis

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Expression of βig-h3 is lower than normal in keratoconus corneas but increases with scarring. / Takács, Lili; Csutak, Adrienne; Balázs, Erzsébet; Módis, L.; Berta, A.

In: Cornea, Vol. 18, No. 5, 09.1999, p. 599-605.

Research output: Contribution to journalArticle

Takács, Lili ; Csutak, Adrienne ; Balázs, Erzsébet ; Módis, L. ; Berta, A. / Expression of βig-h3 is lower than normal in keratoconus corneas but increases with scarring. In: Cornea. 1999 ; Vol. 18, No. 5. pp. 599-605.
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AU - Berta, A.

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