In ruminants maternal immunity is exclusively mediated by colostral immunoglobulins. The authors have previously shown that the bovine neonatal Fc receptor (bFcRn) is expressed not only in the mammary gland but in some other mucosal epithelial cells, which had been previously shown to secrete IgG1. They have also demonstrated that there is a significant difference in the FcRn localization before and after parturition in the mammary acinar cells. Their current studies have demonstrated pH dependent IgG binding in the acinar epithelial cells. The in vitro, radioreceptor assays have shown that similarly to other mammalian species analyzed so far, bovine FcRn binds IgG in a pH dependent manner. Transfection experiments with bFcRn-GFP construct suggest that FcRn is localized intracellularly, mainly in vesicles, like they have previously found in the mammary epithelial cells - that secrete IgG1 into colostrum - before parturition. The current experiments further support their hypothesis that the FcRn is involved in the mammary gland IgG1 transport. Further studies are still requested though, to reveal the mechanism of this IgG transcytosis, and to identify those factors which influence relocalization of the FcRn after parturition.
|Translated title of the contribution||Expression in the udder and IgG binding capacity of bovine neonatal Fc-receptor (bFcRn)|
|Number of pages||8|
|Journal||Magyar Allatorvosok Lapja|
|Publication status||Published - Oct 1 2004|
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