Expression, desensitization, and internalization of the ACTH receptor (MC2R)

Adrian J.L. Clark, Asma H. Baig, Luke Noon, Francesca M. Swords, Laszlo Hunyady, Peter J. King

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Research into the functions and mechanisms of action of the melanocortin 2 receptor (MC2R) has been severely hampered by difficulties in expressing this gene in heterologous cells. This probably arises because of the need for a cofactor for cell surface expression. Using either the Y1 cell line that expresses endogenous MC2R or the Y6 cell line that expresses this putative expression factor, we have explored the mechanisms of desensitization and internalization after agonist stimulation. Protein kinase A dependence of desensitization has been demonstrated, although internalization is apparently independent of this kinase and dependent on a G protein receptor kinase. Possible underlying reasons for this paradox are discussed.

Original languageEnglish
Pages (from-to)111-117
Number of pages7
JournalAnnals of the New York Academy of Sciences
Volume994
DOIs
Publication statusPublished - Jan 1 2003

Keywords

  • Adrenocorticotropin
  • Constitutive activity
  • Desensitization
  • G protein receptor kinase
  • G protein-coupled receptors
  • Melanocortin 2 receptor
  • Molecular chaperones
  • Protein kinase A
  • Receptor internalization

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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