Expression and protective role of heme oxygenase-1 in delayed myocardial preconditioning

Gábor Jancsó, Barbara Cserepes, Balázs Gasz, László Benkó, Balázs Borsiczky, Andrea Ferenc, Mária Kürthy, Boglárka Rácz, János Lantos, János Gal, Endre Arató, Lószló Sínayc, György Wéber, Erzsébet Róth

Research output: Chapter in Book/Report/Conference proceedingConference contribution

42 Citations (Scopus)

Abstract

In the study the authors aimed to demonstrate the expression and protective effect of heme oxygenase-1 (HO-1) in the delayed preconditioning (PC) on cultured myocardiac cells. Neonatal rat cardiac myocytes were exposed to ischemic (ischemic medium [IM] for 20 min) and pharmacological (adenosine, epinephrine, opioid) PC. Twenty-four hours later cells were subjected to a simulated ischemia (SI) - culturing for 3 h in IM, followed by 2-h reperfusion in normal medium - and then lactate dehydrogenase (LDH), live/death ratio, and apoptosis were measured. For demonstrating the protective role of HO-1, its enzymatic activity was competitively inhibited by administration of zinc protoporphyrin IX (ZnPPIX), and HO-1 synthesis was blocked with HO-1 siRNA. Cells in control group were cultured under normoxic conditions. In SI group, cells underwent only an SI without PC. HO-1 expression in all of the groups was demonstrated with immunostaining. Our results showed a significant decrease of LDH release, apoptosis, and cell death in PC groups versus SI group, which has been risen in ZnPPIX- and HO-1 siRNA-treated groups. HO-1 immunostaining showed an appreciable HO-1 expression in PC groups, which was abolished with HO-1 siRNA administration, but not in ZnPPIX group. The results therefore suggest that HO-1 expression increases in both ischemic and pharmacological PC, and HO-1 has cellular protective effect against cell death and apoptosis in ischemia-reperfusion-induced oxidative injury.

Original languageEnglish
Title of host publicationSignal Transduction Pathways, Part C
Subtitle of host publicationCell Signaling in Health and Disease
PublisherBlackwell Publishing Inc.
Pages251-261
Number of pages11
ISBN (Print)1573316954, 9781573316958
DOIs
Publication statusPublished - Jan 2007

Publication series

NameAnnals of the New York Academy of Sciences
Volume1095
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

    Fingerprint

Keywords

  • Heme oxygenase
  • Myocardium
  • Preconditioning

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Jancsó, G., Cserepes, B., Gasz, B., Benkó, L., Borsiczky, B., Ferenc, A., Kürthy, M., Rácz, B., Lantos, J., Gal, J., Arató, E., Sínayc, L., Wéber, G., & Róth, E. (2007). Expression and protective role of heme oxygenase-1 in delayed myocardial preconditioning. In Signal Transduction Pathways, Part C: Cell Signaling in Health and Disease (pp. 251-261). (Annals of the New York Academy of Sciences; Vol. 1095). Blackwell Publishing Inc.. https://doi.org/10.1196/annals.1397.029