Expression and localization of thrombomodulin in preneoplastic bronchial lesions and in lung cancer

E. Tolnay, Thorsten Wiethege, Klaus Michael Müller

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Thrombomodulin (TM) is an endothelial surface glycoprotein that acts as a natural anticoagulant. It inhibits thrombin and accelerates the activation of the anticoagulant protein C. TM has been detected in dermal keratinocytes, where it is associated with terminal differentiation. It can also be detected in various types of squamous malignant neoplasms and in malignancies of endothelial and mesothelial origin, such as Kaposi's sarcoma or malignant mesothelioma, but is absent in pulmonary adenocarcinomas (AC). Seventy-two lung tumour specimens [33 squamous cell carcinomas (SQCC), 23 AC, 1 large cell carcinoma, 8 small cell lung cancers (SCLC) and 7 multidifferentiated tumours (MT)] were analysed immunohistochemically by staining with an anti-TM antibody in order to assess TM expression. All of the SQCC stained positively for TM. In contrast, only 9 AC and 4 MT and none of the SCLC showed positive anti-TM staining. Seven hyperplastic bronchial epithelial specimens and eight preneoplastic bronchial lesions (five cases of moderate dysplasia, two cases of severe dysplasia and one case of carcinoma in situ) were used as controls. Normal or hyperplastic areas of bronchial epithelium revealed no positive reaction. However, a distinct positive anti-TM staining pattern related to the degree of keratiniziation of dysplastic lesions was seen. The present results suggest that anti-TM immunostaining is a useful marker for squamous cell carcinoma in the differential diagnosis of pulmonary carcinoma, also indicating keratinocyte differentiation in dysplastic bronchial epithelium.

Original languageEnglish
Pages (from-to)209-212
Number of pages4
JournalVirchows Archiv
Volume430
Issue number3
DOIs
Publication statusPublished - 1997

Fingerprint

Thrombomodulin
Lung Neoplasms
Squamous Cell Carcinoma
Small Cell Lung Carcinoma
Neoplasms
Staining and Labeling
Keratinocytes
Anticoagulants
Adenocarcinoma
Epithelium
Large Cell Carcinoma
Lung
Kaposi's Sarcoma
Membrane Glycoproteins
Carcinoma in Situ
Protein C
Thrombin
Anti-Idiotypic Antibodies
Differential Diagnosis
Carcinoma

Keywords

  • Epithelial differentiation
  • Keratinisation
  • Lung cancer
  • Preneoplasia
  • Squamous cell carcinoma
  • Thrombomodulin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Expression and localization of thrombomodulin in preneoplastic bronchial lesions and in lung cancer. / Tolnay, E.; Wiethege, Thorsten; Müller, Klaus Michael.

In: Virchows Archiv, Vol. 430, No. 3, 1997, p. 209-212.

Research output: Contribution to journalArticle

Tolnay, E. ; Wiethege, Thorsten ; Müller, Klaus Michael. / Expression and localization of thrombomodulin in preneoplastic bronchial lesions and in lung cancer. In: Virchows Archiv. 1997 ; Vol. 430, No. 3. pp. 209-212.
@article{be5a0f527fce4ae982d1b4fe13fb5eba,
title = "Expression and localization of thrombomodulin in preneoplastic bronchial lesions and in lung cancer",
abstract = "Thrombomodulin (TM) is an endothelial surface glycoprotein that acts as a natural anticoagulant. It inhibits thrombin and accelerates the activation of the anticoagulant protein C. TM has been detected in dermal keratinocytes, where it is associated with terminal differentiation. It can also be detected in various types of squamous malignant neoplasms and in malignancies of endothelial and mesothelial origin, such as Kaposi's sarcoma or malignant mesothelioma, but is absent in pulmonary adenocarcinomas (AC). Seventy-two lung tumour specimens [33 squamous cell carcinomas (SQCC), 23 AC, 1 large cell carcinoma, 8 small cell lung cancers (SCLC) and 7 multidifferentiated tumours (MT)] were analysed immunohistochemically by staining with an anti-TM antibody in order to assess TM expression. All of the SQCC stained positively for TM. In contrast, only 9 AC and 4 MT and none of the SCLC showed positive anti-TM staining. Seven hyperplastic bronchial epithelial specimens and eight preneoplastic bronchial lesions (five cases of moderate dysplasia, two cases of severe dysplasia and one case of carcinoma in situ) were used as controls. Normal or hyperplastic areas of bronchial epithelium revealed no positive reaction. However, a distinct positive anti-TM staining pattern related to the degree of keratiniziation of dysplastic lesions was seen. The present results suggest that anti-TM immunostaining is a useful marker for squamous cell carcinoma in the differential diagnosis of pulmonary carcinoma, also indicating keratinocyte differentiation in dysplastic bronchial epithelium.",
keywords = "Epithelial differentiation, Keratinisation, Lung cancer, Preneoplasia, Squamous cell carcinoma, Thrombomodulin",
author = "E. Tolnay and Thorsten Wiethege and M{\"u}ller, {Klaus Michael}",
year = "1997",
doi = "10.1007/BF01324803",
language = "English",
volume = "430",
pages = "209--212",
journal = "Virchows Archiv - A Pathological Anatomy and Histopathology",
issn = "0945-6317",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Expression and localization of thrombomodulin in preneoplastic bronchial lesions and in lung cancer

AU - Tolnay, E.

AU - Wiethege, Thorsten

AU - Müller, Klaus Michael

PY - 1997

Y1 - 1997

N2 - Thrombomodulin (TM) is an endothelial surface glycoprotein that acts as a natural anticoagulant. It inhibits thrombin and accelerates the activation of the anticoagulant protein C. TM has been detected in dermal keratinocytes, where it is associated with terminal differentiation. It can also be detected in various types of squamous malignant neoplasms and in malignancies of endothelial and mesothelial origin, such as Kaposi's sarcoma or malignant mesothelioma, but is absent in pulmonary adenocarcinomas (AC). Seventy-two lung tumour specimens [33 squamous cell carcinomas (SQCC), 23 AC, 1 large cell carcinoma, 8 small cell lung cancers (SCLC) and 7 multidifferentiated tumours (MT)] were analysed immunohistochemically by staining with an anti-TM antibody in order to assess TM expression. All of the SQCC stained positively for TM. In contrast, only 9 AC and 4 MT and none of the SCLC showed positive anti-TM staining. Seven hyperplastic bronchial epithelial specimens and eight preneoplastic bronchial lesions (five cases of moderate dysplasia, two cases of severe dysplasia and one case of carcinoma in situ) were used as controls. Normal or hyperplastic areas of bronchial epithelium revealed no positive reaction. However, a distinct positive anti-TM staining pattern related to the degree of keratiniziation of dysplastic lesions was seen. The present results suggest that anti-TM immunostaining is a useful marker for squamous cell carcinoma in the differential diagnosis of pulmonary carcinoma, also indicating keratinocyte differentiation in dysplastic bronchial epithelium.

AB - Thrombomodulin (TM) is an endothelial surface glycoprotein that acts as a natural anticoagulant. It inhibits thrombin and accelerates the activation of the anticoagulant protein C. TM has been detected in dermal keratinocytes, where it is associated with terminal differentiation. It can also be detected in various types of squamous malignant neoplasms and in malignancies of endothelial and mesothelial origin, such as Kaposi's sarcoma or malignant mesothelioma, but is absent in pulmonary adenocarcinomas (AC). Seventy-two lung tumour specimens [33 squamous cell carcinomas (SQCC), 23 AC, 1 large cell carcinoma, 8 small cell lung cancers (SCLC) and 7 multidifferentiated tumours (MT)] were analysed immunohistochemically by staining with an anti-TM antibody in order to assess TM expression. All of the SQCC stained positively for TM. In contrast, only 9 AC and 4 MT and none of the SCLC showed positive anti-TM staining. Seven hyperplastic bronchial epithelial specimens and eight preneoplastic bronchial lesions (five cases of moderate dysplasia, two cases of severe dysplasia and one case of carcinoma in situ) were used as controls. Normal or hyperplastic areas of bronchial epithelium revealed no positive reaction. However, a distinct positive anti-TM staining pattern related to the degree of keratiniziation of dysplastic lesions was seen. The present results suggest that anti-TM immunostaining is a useful marker for squamous cell carcinoma in the differential diagnosis of pulmonary carcinoma, also indicating keratinocyte differentiation in dysplastic bronchial epithelium.

KW - Epithelial differentiation

KW - Keratinisation

KW - Lung cancer

KW - Preneoplasia

KW - Squamous cell carcinoma

KW - Thrombomodulin

UR - http://www.scopus.com/inward/record.url?scp=0030969332&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030969332&partnerID=8YFLogxK

U2 - 10.1007/BF01324803

DO - 10.1007/BF01324803

M3 - Article

C2 - 9099977

AN - SCOPUS:0030969332

VL - 430

SP - 209

EP - 212

JO - Virchows Archiv - A Pathological Anatomy and Histopathology

JF - Virchows Archiv - A Pathological Anatomy and Histopathology

SN - 0945-6317

IS - 3

ER -