Expression and functional studies on the noncoding RNA, PRINS

Krisztina Szegedi, Anikó Göblös, Sarolta Bacsa, Mária Antal, István Balázs Németh, Zsuzsanna Bata-Csörgo, Lajos Kemény, Attila Dobozy, Márta Széll

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

PRINS, a noncoding RNA identified earlier by our research group, contributes to psoriasis susceptibility and cellular stress response. We have now studied the cellular and histological distribution of PRINS by using in situ hybridization and demonstrated variable expressions in different human tissues and a consistent staining pattern in epidermal keratinocytes and in vitro cultured keratinocytes. To identify the cellular function(s) of PRINS, we searched for a direct interacting partner(s) of this stress-induced molecule. In HaCaT and NHEK cell lysates, the protein proved to be nucleophosmin (NPM) protein as a potential physical interactor with PRINS. Immunohistochemical experiments revealed an elevated expression of NPM in the dividing cells of the basal layers of psoriatic involved skin samples as compared with healthy and psoriatic uninvolved samples. Others have previously shown that NPM is a ubiquitously expressed nucleolar phosphoprotein which shuttles to the nucleoplasm after UV-B irradiation in fibroblasts and cancer cells. We detected a similar translocation of NPM in UV-B-irradiated cultured keratinocytes. The gene-specific silencing of PRINS resulted in the retention of NPM in the nucleolus of UV-B-irradiated keratinocytes; suggesting that PRINS may play a role in the NPM-mediated cellular stress response in the skin.

Original languageEnglish
Pages (from-to)205-225
Number of pages21
JournalInternational journal of molecular sciences
Volume14
Issue number1
DOIs
Publication statusPublished - Jan 2013

Keywords

  • Keratinocyte proliferation
  • Noncoding RNA
  • Nucleophosmin
  • Psoriasis
  • UV-B irradiation

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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