Expression and characterization of the olfactomedin domain of human myocilin

Ildikó Nagy, M. Trexler, L. Patthy

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The olfactomedin-domain has been first identified in olfactomedin, an extracellular matrix protein of the olfactory neuroepithelium. Members of this extracellular domain-family have since been shown to be present in several metazoan proteins, such as latrophilins, myocilins, and noelins, but their biological function is unknown. The olfactomedin-domain of myocilin is of considerable interest, since mutations affecting this domain are associated with primary open angle glaucoma. In order to define structural features of this domain-type we have expressed the olfactomedin-domain of human myocilin in Pichia pastoris. The olfactomedin-domain contains a single disulphide-bond connecting Cys-245 and Cys-433 residues; secondary structure predictions and circular dichroism studies indicate that it consists primarily of β-strands. It is noteworthy that the majority of mutations associated with severe forms of glaucoma affect residues that reside in conserved secondary structural elements of the olfactomedin-domain or are otherwise critical for the integrity of this protein-fold.

Original languageEnglish
Pages (from-to)554-561
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume302
Issue number3
DOIs
Publication statusPublished - Mar 14 2003

Fingerprint

Mutation
Pichia
Extracellular Matrix Proteins
Circular Dichroism
Disulfides
Glaucoma
trabecular meshwork-induced glucocorticoid response protein
olfactomedin
Proteins
Primary Open Angle Glaucoma
alpha-latrotoxin receptor
single bond

Keywords

  • Circular dichroism spectroscopy
  • Glaucoma
  • Latrophilin
  • Myocilin
  • Noelin
  • Olfactomedin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Expression and characterization of the olfactomedin domain of human myocilin. / Nagy, Ildikó; Trexler, M.; Patthy, L.

In: Biochemical and Biophysical Research Communications, Vol. 302, No. 3, 14.03.2003, p. 554-561.

Research output: Contribution to journalArticle

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