Expression and activation of Akt/protein kinase B in sexually immature and mature rat uterus

Ferenc Lengyel, Zsuzsanna Vértes, Kálmán A. Kovács, József L. Környei, Balázs Sumegi, Marietta Vértes

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10 Citations (Scopus)

Abstract

This study investigated the expression and activation of Akt/PKB in developing and adult rat uterus. Expression of Akt was observed in uteri from adult ovariectomized and 7-35-day-old rats and no changes were observed in response to in vivo estradiol treatment (1-100 μg/100 g b.w.). To examine the mechanisms of PKB/Akt activation, phosphorylation at Thr 308 and Ser 473 regulatory sites were studied in uteri. Akt was constitutively phosphorylated on Ser 473 residue in the untreated, control uteri, while phosphorylation of Thr 308 was observed only after estradiol 17β (E2) treatment. The effects of E2 treatment were age dependent, no response was induced in 11-day-old uteri, while in 28 days and older rats the activation of Akt at both regulatory sites, Ser 473 and Thr 308, increased, the first response was detected 2 h after treatment, reaching the highest rate at 6 h. The rate of phosphorylation was stronger at Ser 473 residue. The results suggest that the regulation of Akt activation at two regulatory sites in rat uteri are different, phosphorylation of Thr 308 seems to be entirely estrogen dependent, while the phosphorylation of Ser 473 is regulated by other factors as well as estrogen.

Original languageEnglish
Pages (from-to)285-288
Number of pages4
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume91
Issue number4-5
DOIs
Publication statusPublished - Aug 1 2004

Keywords

  • Akt
  • Development
  • Estradiol
  • In vivo
  • Rat uterus

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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