Expression analysis of KCS genes potentially involved in cuticular wax production in the apple cultivar 'Gegesi Zöld'

Zs Albert, B. Ivanics, A. Molnár, Cs Deák, A. Miskó, I. Papp, M. Tóth

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The cuticle plays important roles in plants' life, from controlling non-stomatal transpiration, through plant-pathogen interactions, to taking part in plant development. In case of the model plant Arabidopsis thaliana, the biosynthetic pathways of cuticular lipids are well-studied, and at least partially understood. It is known that an elongation complex outside the chloroplast extends plastidial C16 or C18 chain length fatty acids. The resulting very long chain fatty acid will be modified to different lipoid compounds that finally load on the outer surface of the cuticle. A battery of enzymes has been identified performing chain elongation, modifications and transport of the lipid molecules. In our experiments, we wished to identify some KCS gene homologs in apple, potentially involved in these processes, and analyze expression of the candidate genes in different fruit tissues. Based on data from the literature dealing with the Arabidopsis model we performed in silico analysis of apple EST as well as genome sequences and found candidate KCS genes that could be related to wax production. Subsequent RT-PCR analysis of most of these sequences showed them to be expressed in the fruit, furthermore in some cases the expression was peel-specific. This makes those sequences likely candidates for apple genes involved in fruit cuticular wax production.

Original languageEnglish
Title of host publicationActa Horticulturae
Pages205-208
Number of pages4
Volume981
Edition1
Publication statusPublished - Mar 31 2012

Publication series

NameActa Horticulturae
Number1
Volume981
ISSN (Print)05677572

Fingerprint

epicuticular wax
apples
fruits
cultivars
very long chain fatty acids
genes
lipids
waxes
plant pathogens
plant development
biochemical pathways
transpiration
Arabidopsis thaliana
chloroplasts
reverse transcriptase polymerase chain reaction
Arabidopsis
fatty acids
gene expression
genome
enzymes

Keywords

  • Apple
  • Cuticle
  • Gene expression
  • Wax

ASJC Scopus subject areas

  • Horticulture

Cite this

Albert, Z., Ivanics, B., Molnár, A., Deák, C., Miskó, A., Papp, I., & Tóth, M. (2012). Expression analysis of KCS genes potentially involved in cuticular wax production in the apple cultivar 'Gegesi Zöld'. In Acta Horticulturae (1 ed., Vol. 981, pp. 205-208). (Acta Horticulturae; Vol. 981, No. 1).

Expression analysis of KCS genes potentially involved in cuticular wax production in the apple cultivar 'Gegesi Zöld'. / Albert, Zs; Ivanics, B.; Molnár, A.; Deák, Cs; Miskó, A.; Papp, I.; Tóth, M.

Acta Horticulturae. Vol. 981 1. ed. 2012. p. 205-208 (Acta Horticulturae; Vol. 981, No. 1).

Research output: Chapter in Book/Report/Conference proceedingChapter

Albert, Z, Ivanics, B, Molnár, A, Deák, C, Miskó, A, Papp, I & Tóth, M 2012, Expression analysis of KCS genes potentially involved in cuticular wax production in the apple cultivar 'Gegesi Zöld'. in Acta Horticulturae. 1 edn, vol. 981, Acta Horticulturae, no. 1, vol. 981, pp. 205-208.
Albert Z, Ivanics B, Molnár A, Deák C, Miskó A, Papp I et al. Expression analysis of KCS genes potentially involved in cuticular wax production in the apple cultivar 'Gegesi Zöld'. In Acta Horticulturae. 1 ed. Vol. 981. 2012. p. 205-208. (Acta Horticulturae; 1).
Albert, Zs ; Ivanics, B. ; Molnár, A. ; Deák, Cs ; Miskó, A. ; Papp, I. ; Tóth, M. / Expression analysis of KCS genes potentially involved in cuticular wax production in the apple cultivar 'Gegesi Zöld'. Acta Horticulturae. Vol. 981 1. ed. 2012. pp. 205-208 (Acta Horticulturae; 1).
@inbook{c8ed89e6a0de4e4abeb87538a120786f,
title = "Expression analysis of KCS genes potentially involved in cuticular wax production in the apple cultivar 'Gegesi Z{\"o}ld'",
abstract = "The cuticle plays important roles in plants' life, from controlling non-stomatal transpiration, through plant-pathogen interactions, to taking part in plant development. In case of the model plant Arabidopsis thaliana, the biosynthetic pathways of cuticular lipids are well-studied, and at least partially understood. It is known that an elongation complex outside the chloroplast extends plastidial C16 or C18 chain length fatty acids. The resulting very long chain fatty acid will be modified to different lipoid compounds that finally load on the outer surface of the cuticle. A battery of enzymes has been identified performing chain elongation, modifications and transport of the lipid molecules. In our experiments, we wished to identify some KCS gene homologs in apple, potentially involved in these processes, and analyze expression of the candidate genes in different fruit tissues. Based on data from the literature dealing with the Arabidopsis model we performed in silico analysis of apple EST as well as genome sequences and found candidate KCS genes that could be related to wax production. Subsequent RT-PCR analysis of most of these sequences showed them to be expressed in the fruit, furthermore in some cases the expression was peel-specific. This makes those sequences likely candidates for apple genes involved in fruit cuticular wax production.",
keywords = "Apple, Cuticle, Gene expression, Wax",
author = "Zs Albert and B. Ivanics and A. Moln{\'a}r and Cs De{\'a}k and A. Misk{\'o} and I. Papp and M. T{\'o}th",
year = "2012",
month = "3",
day = "31",
language = "English",
isbn = "9789066051881",
volume = "981",
series = "Acta Horticulturae",
number = "1",
pages = "205--208",
booktitle = "Acta Horticulturae",
edition = "1",

}

TY - CHAP

T1 - Expression analysis of KCS genes potentially involved in cuticular wax production in the apple cultivar 'Gegesi Zöld'

AU - Albert, Zs

AU - Ivanics, B.

AU - Molnár, A.

AU - Deák, Cs

AU - Miskó, A.

AU - Papp, I.

AU - Tóth, M.

PY - 2012/3/31

Y1 - 2012/3/31

N2 - The cuticle plays important roles in plants' life, from controlling non-stomatal transpiration, through plant-pathogen interactions, to taking part in plant development. In case of the model plant Arabidopsis thaliana, the biosynthetic pathways of cuticular lipids are well-studied, and at least partially understood. It is known that an elongation complex outside the chloroplast extends plastidial C16 or C18 chain length fatty acids. The resulting very long chain fatty acid will be modified to different lipoid compounds that finally load on the outer surface of the cuticle. A battery of enzymes has been identified performing chain elongation, modifications and transport of the lipid molecules. In our experiments, we wished to identify some KCS gene homologs in apple, potentially involved in these processes, and analyze expression of the candidate genes in different fruit tissues. Based on data from the literature dealing with the Arabidopsis model we performed in silico analysis of apple EST as well as genome sequences and found candidate KCS genes that could be related to wax production. Subsequent RT-PCR analysis of most of these sequences showed them to be expressed in the fruit, furthermore in some cases the expression was peel-specific. This makes those sequences likely candidates for apple genes involved in fruit cuticular wax production.

AB - The cuticle plays important roles in plants' life, from controlling non-stomatal transpiration, through plant-pathogen interactions, to taking part in plant development. In case of the model plant Arabidopsis thaliana, the biosynthetic pathways of cuticular lipids are well-studied, and at least partially understood. It is known that an elongation complex outside the chloroplast extends plastidial C16 or C18 chain length fatty acids. The resulting very long chain fatty acid will be modified to different lipoid compounds that finally load on the outer surface of the cuticle. A battery of enzymes has been identified performing chain elongation, modifications and transport of the lipid molecules. In our experiments, we wished to identify some KCS gene homologs in apple, potentially involved in these processes, and analyze expression of the candidate genes in different fruit tissues. Based on data from the literature dealing with the Arabidopsis model we performed in silico analysis of apple EST as well as genome sequences and found candidate KCS genes that could be related to wax production. Subsequent RT-PCR analysis of most of these sequences showed them to be expressed in the fruit, furthermore in some cases the expression was peel-specific. This makes those sequences likely candidates for apple genes involved in fruit cuticular wax production.

KW - Apple

KW - Cuticle

KW - Gene expression

KW - Wax

UR - http://www.scopus.com/inward/record.url?scp=84879222228&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879222228&partnerID=8YFLogxK

M3 - Chapter

SN - 9789066051881

VL - 981

T3 - Acta Horticulturae

SP - 205

EP - 208

BT - Acta Horticulturae

ER -