Expression analysis of heat shock genes in the skin, spleen and blood of common carp (Cyprinus carpio) after cadmium exposure and hypothermia

Ágnes Ferencz, Renáta Juhász, Monica Butnariu, K. Aranka Deér, I. Varga, J. Nemcsók

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Heat shock proteins are chaperones that play a pivotal role in controling multiple regulatory pathways such as stress defense, hormone signaling, cell cycle control, cell proliferation and differentiation, and apoptosis. In this study, the expression patterns of four well-known heat shock genes (hsp70, hsc70-1, hsc70-2 and hsp90α) were characterized in the skin, spleen and blood cells of the common carp, under unstressed conditions and after Cd 2+ treatment or hypothermia. The examined genes were expressed in a tissue-specific manner: hsc70-2 was expressed constitutively, and was at best only slightly inducible; hsp90α exhibited a high basic expression in all three tissues, whereas hsc70-1 did so only in the blood cells, the expression of hsp70 proved to be below the level of detection in unstressed fish. Cold shock induced the expression of hsp genes in the spleen (hsp90α) and blood cells (hsp70, hsc70-1 and hsp90α), while Cd 2+ treatment has no effect on the expression pattern. The highest inducibilities were detected in the skin: for hsp70 an induction of at least 20-fold after cadmium exposure, for hsc70-1 of at least 30-fold and for hsp90α of 3-fold after hypothermia.

Original languageEnglish
Pages (from-to)15-25
Number of pages11
JournalActa Biologica Hungarica
Volume63
Issue number1
DOIs
Publication statusPublished - Mar 1 2012

Fingerprint

Hypothermia
Carps
heat shock
Cadmium
Shock
skin
Blood Cells
Skin
Blood
cadmium
Spleen
Hot Temperature
Genes
blood
Cells
fold
gene
Tissue
Cell signaling
apoptosis

Keywords

  • Cadmium treatment
  • carp
  • heat shock genes
  • RT-PCR

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Cite this

Expression analysis of heat shock genes in the skin, spleen and blood of common carp (Cyprinus carpio) after cadmium exposure and hypothermia. / Ferencz, Ágnes; Juhász, Renáta; Butnariu, Monica; Deér, K. Aranka; Varga, I.; Nemcsók, J.

In: Acta Biologica Hungarica, Vol. 63, No. 1, 01.03.2012, p. 15-25.

Research output: Contribution to journalArticle

Ferencz, Ágnes ; Juhász, Renáta ; Butnariu, Monica ; Deér, K. Aranka ; Varga, I. ; Nemcsók, J. / Expression analysis of heat shock genes in the skin, spleen and blood of common carp (Cyprinus carpio) after cadmium exposure and hypothermia. In: Acta Biologica Hungarica. 2012 ; Vol. 63, No. 1. pp. 15-25.
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