Exposure to urban PM1 in rats

Development of bronchial inflammation and airway hyperresponsiveness

Ágnes Filep, Gergely H. Fodor, Fruzsina Kun-Szabó, L. Tiszlavicz, Zsolt Rázga, Gábor Bozsó, Z. Bozóki, Gábor Szabó, F. Peták

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: Several epidemiological and laboratory studies have evidenced the fact that atmospheric particulate matter (PM) increases the risk of respiratory morbidity. It is well known that the smallest fraction of PM (PM1 - particulate matter having a diameter below 1 μm) penetrates the deepest into the airways. The ratio of the different size fractions in PM is highly variable, but in industrial areas PM1 can be significant. Despite these facts, the health effects of PM1 have been poorly investigated and air quality standards are based on PM10 and PM2.5 (PM having diameters below 10 μm and 2.5 μm, respectively) concentrations. Therefore, this study aimed at determining whether exposure to ambient PM1 at a near alert threshold level for PM10 has respiratory consequences in rats. Methods: Rats were either exposed for 6 weeks to 100 μg/m3 (alert threshold level for PM10 in Hungary) urban submicron aerosol, or were kept in room air. End-expiratory lung volume, airway resistance (Raw) and respiratory tissue mechanics were measured. Respiratory mechanics were measured under baseline conditions and following intravenous methacholine challenges to characterize the development of airway hyperresponsiveness (AH). Bronchoalveolar lavage fluid (BALF) was analyzed and lung histology was performed. Results: No significant differences were detected in lung volume and mechanical parameters at baseline. However, the exposed rats exhibited significantly greater MCh-induced responses in Raw, demonstrating the progression of AH. The associated bronchial inflammation was evidenced by the accumulation of inflammatory cells in BALF and by lung histology. Conclusions: Our findings suggest that exposure to concentrated ambient PM1 (mass concentration at the threshold level for PM10) leads to the development of mild respiratory symptoms in healthy adult rats, which may suggest a need for the reconsideration of threshold limits for airborne PM1.

Original languageEnglish
Article number26
JournalRespiratory Research
Volume17
Issue number1
DOIs
Publication statusPublished - Mar 10 2016

Fingerprint

Particulate Matter
Inflammation
Respiratory Mechanics
Lung
Bronchoalveolar Lavage Fluid
Histology
Air
Airway Resistance
Methacholine Chloride
Hungary
Aerosols
Epidemiologic Studies
Morbidity
Health

Keywords

  • Air pollution
  • Airway hyperresponsiveness
  • Ambient aerosol
  • Bronchial inflammation
  • Lung function

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Exposure to urban PM1 in rats : Development of bronchial inflammation and airway hyperresponsiveness. / Filep, Ágnes; Fodor, Gergely H.; Kun-Szabó, Fruzsina; Tiszlavicz, L.; Rázga, Zsolt; Bozsó, Gábor; Bozóki, Z.; Szabó, Gábor; Peták, F.

In: Respiratory Research, Vol. 17, No. 1, 26, 10.03.2016.

Research output: Contribution to journalArticle

Filep, Ágnes ; Fodor, Gergely H. ; Kun-Szabó, Fruzsina ; Tiszlavicz, L. ; Rázga, Zsolt ; Bozsó, Gábor ; Bozóki, Z. ; Szabó, Gábor ; Peták, F. / Exposure to urban PM1 in rats : Development of bronchial inflammation and airway hyperresponsiveness. In: Respiratory Research. 2016 ; Vol. 17, No. 1.
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AU - Tiszlavicz, L.

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AB - Background: Several epidemiological and laboratory studies have evidenced the fact that atmospheric particulate matter (PM) increases the risk of respiratory morbidity. It is well known that the smallest fraction of PM (PM1 - particulate matter having a diameter below 1 μm) penetrates the deepest into the airways. The ratio of the different size fractions in PM is highly variable, but in industrial areas PM1 can be significant. Despite these facts, the health effects of PM1 have been poorly investigated and air quality standards are based on PM10 and PM2.5 (PM having diameters below 10 μm and 2.5 μm, respectively) concentrations. Therefore, this study aimed at determining whether exposure to ambient PM1 at a near alert threshold level for PM10 has respiratory consequences in rats. Methods: Rats were either exposed for 6 weeks to 100 μg/m3 (alert threshold level for PM10 in Hungary) urban submicron aerosol, or were kept in room air. End-expiratory lung volume, airway resistance (Raw) and respiratory tissue mechanics were measured. Respiratory mechanics were measured under baseline conditions and following intravenous methacholine challenges to characterize the development of airway hyperresponsiveness (AH). Bronchoalveolar lavage fluid (BALF) was analyzed and lung histology was performed. Results: No significant differences were detected in lung volume and mechanical parameters at baseline. However, the exposed rats exhibited significantly greater MCh-induced responses in Raw, demonstrating the progression of AH. The associated bronchial inflammation was evidenced by the accumulation of inflammatory cells in BALF and by lung histology. Conclusions: Our findings suggest that exposure to concentrated ambient PM1 (mass concentration at the threshold level for PM10) leads to the development of mild respiratory symptoms in healthy adult rats, which may suggest a need for the reconsideration of threshold limits for airborne PM1.

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