Exposure of tumor necrosis factor‐α to luminal membrane of bovine brain capillary endothelial cells cocultured with astrocytes induces a delayed increase of permeability and cytoplasmic stress fiber formation of actin

M. A. Deli, L. Descamps, M. ‐P Dehouck, R. Cecchelli, F. Joó, C. S. Ábrahám, G. Torpier

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

Tumor necrosis factor‐α (TNF‐α), a proinflammatory cytokine, has long been known to be involved in the pathogenesis of central nervous system infections and of certain neurodegenerative diseases. However, the possible role of the blood‐brain barrier (BBB), the active interface between the blood circulation and brain tissue, remained unknown during these pathological conditions. In our in vitro reconstructed BBB model, 1‐hr exposure of recombinant human TNF‐α (in concentrations of 50, 250, and 500 U/ml, respectively) to the luminal membrane of bovine brain capillary endothelial cells (BBCEC) did not change significantly the transendothelia: flux of either sucrose (m.w. 342 Da), or inulin (m.w. 5 kDa) up to 4 hr (early phase), except for a slight decrease (P< 0.05) in sucrose permeation at 2–4 hr with the highest dose of TNF‐α On the other hand, at 16 hr after the 1‐hr challenge with TNF‐α (delayed phase) at all 3 concentrations, significant increase was induced in the permeability of BBCEC monolayers for both markers. These changes of permeability were accompanied by a selective reorganization of F‐actin filaments into stress fibers, while the intracellular distribution of vimentin remained similar to the control. These results suggest that BBCEC can respond directly to TNF‐α by a delayed increase of permeability and reorganization of actin filaments. ©1995 Wiley‐Liss, Inc.

Original languageEnglish
Pages (from-to)717-726
Number of pages10
JournalJournal of Neuroscience Research
Volume41
Issue number6
DOIs
Publication statusPublished - Aug 15 1995

Keywords

  • actin
  • astrocytes
  • blood‐brain barrier (BBB)
  • endothelial cells
  • stress fiber
  • tumor necrosis factor‐α
  • vascular permeability

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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