Exploring the role of the phage-specific insert of bacteriophage Φ11 dUTPase

Kinga Nyíri, Veronika Papp-Kádár, Judit E. Szabó, Veronika Németh, B. Vértessy

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

dUTPases are essential for maintaining genome integrity. Recently, in the case of a dUTPase from a Staphylococcal phage, another different physiological function was also suggested. Namely, it was shown that dUTPase from the Staphylococcus aureus bacteriophage 11 is capable of binding to the Staphylococcal Stl repressor protein. This binding interferes with the function of Stl. In the present study, we investigated the putative role of a phage dUTPase-specific peptide segment in the interaction of dUTPase with Stl and in impeding Stl-DNA complex formation. We show that dUTPase from Mycobacterium tuberculosis that lacks the phage-specific insert is also capable of disrupting the complexation between Stl and DNA. Hence, the insert segment is not essential for perturbation of the Stl function. However, we also demonstrate that in case of the phage dUTPase, the insert-lacking construct is deficient in perturbation of Stl activity. These findings clearly indicate that the phagespecific insert has a well-defined role only in the context of the phage dUTPase.

Original languageEnglish
Pages (from-to)1425-1432
Number of pages8
JournalStructural Chemistry
Volume26
Issue number5-6
DOIs
Publication statusPublished - Aug 30 2015

Fingerprint

bacteriophages
Bacteriophages
inserts
deoxyribonucleic acid
tuberculosis
disrupting
perturbation
staphylococcus
genome
integrity
peptides
Repressor Proteins
proteins
DNA
dUTP pyrophosphatase
Complexation
Genes
interactions
Peptides

Keywords

  • dUTPase
  • Non-canonical insert
  • Protein-DNA interaction
  • Protein-protein interaction
  • Staphylococcal repressor

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

Cite this

Exploring the role of the phage-specific insert of bacteriophage Φ11 dUTPase. / Nyíri, Kinga; Papp-Kádár, Veronika; Szabó, Judit E.; Németh, Veronika; Vértessy, B.

In: Structural Chemistry, Vol. 26, No. 5-6, 30.08.2015, p. 1425-1432.

Research output: Contribution to journalArticle

Nyíri, Kinga ; Papp-Kádár, Veronika ; Szabó, Judit E. ; Németh, Veronika ; Vértessy, B. / Exploring the role of the phage-specific insert of bacteriophage Φ11 dUTPase. In: Structural Chemistry. 2015 ; Vol. 26, No. 5-6. pp. 1425-1432.
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