Exploiting fluorescence for multiplex immunoassays on protein microarrays

Melinda Herbáth, Krisztián Papp, Andrea Balogh, J. Matkó, J. Prechl

Research output: Contribution to journalReview article

6 Citations (Scopus)

Abstract

Protein microarray technology is becoming the method of choice for identifying protein interaction partners, detecting specific proteins, carbohydrates and lipids, or for characterizing protein interactions and serum antibodies in a massively parallel manner. Availability of the well-established instrumentation of DNA arrays and development of new fluorescent detection instruments promoted the spread of this technique. Fluorescent detection has the advantage of high sensitivity, specificity, simplicity and wide dynamic range required by most measurements. Fluorescence through specifically designed probes and an increasing variety of detection modes offers an excellent tool for such microarray platforms. Measuring for example the level of antibodies, their isotypes and/or antigen specificity simultaneously can offer more complex and comprehensive information about the investigated biological phenomenon, especially if we take into consideration that hundreds of samples can be measured in a single assay. Not only body fluids, but also cell lysates, extracted cellular components, and intact living cells can be analyzed on protein arrays for monitoring functional responses to printed samples on the surface. As a rapidly evolving area, protein microarray technology offers a great bulk of information and new depth of knowledge. These are the features that endow protein arrays with wide applicability and robust sample analyzing capability. On the whole, protein arrays are emerging new tools not just in proteomics, but glycomics, lipidomics, and are also important for immunological research. In this review we attempt to summarize the technical aspects of planar fluorescent microarray technology along with the description of its main immunological applications.

Original languageEnglish
Article number032001
JournalMethods and Applications in Fluorescence
Volume2
Issue number3
DOIs
Publication statusPublished - Sep 1 2014

Fingerprint

immunoassay
Microarrays
Fluorescence
proteins
Proteins
fluorescence
antibodies
Antibodies
body fluids
Body fluids
carbohydrates
antigens
Antigens
Carbohydrates
serums
Lipids
dynamic range
availability
lipids
emerging

Keywords

  • Immunoassay
  • Multiplex
  • Protein microarray

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Spectroscopy
  • Materials Science(all)

Cite this

Exploiting fluorescence for multiplex immunoassays on protein microarrays. / Herbáth, Melinda; Papp, Krisztián; Balogh, Andrea; Matkó, J.; Prechl, J.

In: Methods and Applications in Fluorescence, Vol. 2, No. 3, 032001, 01.09.2014.

Research output: Contribution to journalReview article

@article{a09e166e9a794bee935121934f11f18e,
title = "Exploiting fluorescence for multiplex immunoassays on protein microarrays",
abstract = "Protein microarray technology is becoming the method of choice for identifying protein interaction partners, detecting specific proteins, carbohydrates and lipids, or for characterizing protein interactions and serum antibodies in a massively parallel manner. Availability of the well-established instrumentation of DNA arrays and development of new fluorescent detection instruments promoted the spread of this technique. Fluorescent detection has the advantage of high sensitivity, specificity, simplicity and wide dynamic range required by most measurements. Fluorescence through specifically designed probes and an increasing variety of detection modes offers an excellent tool for such microarray platforms. Measuring for example the level of antibodies, their isotypes and/or antigen specificity simultaneously can offer more complex and comprehensive information about the investigated biological phenomenon, especially if we take into consideration that hundreds of samples can be measured in a single assay. Not only body fluids, but also cell lysates, extracted cellular components, and intact living cells can be analyzed on protein arrays for monitoring functional responses to printed samples on the surface. As a rapidly evolving area, protein microarray technology offers a great bulk of information and new depth of knowledge. These are the features that endow protein arrays with wide applicability and robust sample analyzing capability. On the whole, protein arrays are emerging new tools not just in proteomics, but glycomics, lipidomics, and are also important for immunological research. In this review we attempt to summarize the technical aspects of planar fluorescent microarray technology along with the description of its main immunological applications.",
keywords = "Immunoassay, Multiplex, Protein microarray",
author = "Melinda Herb{\'a}th and Kriszti{\'a}n Papp and Andrea Balogh and J. Matk{\'o} and J. Prechl",
year = "2014",
month = "9",
day = "1",
doi = "10.1088/2050-6120/2/3/032001",
language = "English",
volume = "2",
journal = "Methods and Applications in Fluorescence",
issn = "2050-6120",
publisher = "IOP Publishing Ltd.",
number = "3",

}

TY - JOUR

T1 - Exploiting fluorescence for multiplex immunoassays on protein microarrays

AU - Herbáth, Melinda

AU - Papp, Krisztián

AU - Balogh, Andrea

AU - Matkó, J.

AU - Prechl, J.

PY - 2014/9/1

Y1 - 2014/9/1

N2 - Protein microarray technology is becoming the method of choice for identifying protein interaction partners, detecting specific proteins, carbohydrates and lipids, or for characterizing protein interactions and serum antibodies in a massively parallel manner. Availability of the well-established instrumentation of DNA arrays and development of new fluorescent detection instruments promoted the spread of this technique. Fluorescent detection has the advantage of high sensitivity, specificity, simplicity and wide dynamic range required by most measurements. Fluorescence through specifically designed probes and an increasing variety of detection modes offers an excellent tool for such microarray platforms. Measuring for example the level of antibodies, their isotypes and/or antigen specificity simultaneously can offer more complex and comprehensive information about the investigated biological phenomenon, especially if we take into consideration that hundreds of samples can be measured in a single assay. Not only body fluids, but also cell lysates, extracted cellular components, and intact living cells can be analyzed on protein arrays for monitoring functional responses to printed samples on the surface. As a rapidly evolving area, protein microarray technology offers a great bulk of information and new depth of knowledge. These are the features that endow protein arrays with wide applicability and robust sample analyzing capability. On the whole, protein arrays are emerging new tools not just in proteomics, but glycomics, lipidomics, and are also important for immunological research. In this review we attempt to summarize the technical aspects of planar fluorescent microarray technology along with the description of its main immunological applications.

AB - Protein microarray technology is becoming the method of choice for identifying protein interaction partners, detecting specific proteins, carbohydrates and lipids, or for characterizing protein interactions and serum antibodies in a massively parallel manner. Availability of the well-established instrumentation of DNA arrays and development of new fluorescent detection instruments promoted the spread of this technique. Fluorescent detection has the advantage of high sensitivity, specificity, simplicity and wide dynamic range required by most measurements. Fluorescence through specifically designed probes and an increasing variety of detection modes offers an excellent tool for such microarray platforms. Measuring for example the level of antibodies, their isotypes and/or antigen specificity simultaneously can offer more complex and comprehensive information about the investigated biological phenomenon, especially if we take into consideration that hundreds of samples can be measured in a single assay. Not only body fluids, but also cell lysates, extracted cellular components, and intact living cells can be analyzed on protein arrays for monitoring functional responses to printed samples on the surface. As a rapidly evolving area, protein microarray technology offers a great bulk of information and new depth of knowledge. These are the features that endow protein arrays with wide applicability and robust sample analyzing capability. On the whole, protein arrays are emerging new tools not just in proteomics, but glycomics, lipidomics, and are also important for immunological research. In this review we attempt to summarize the technical aspects of planar fluorescent microarray technology along with the description of its main immunological applications.

KW - Immunoassay

KW - Multiplex

KW - Protein microarray

UR - http://www.scopus.com/inward/record.url?scp=84974560600&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84974560600&partnerID=8YFLogxK

U2 - 10.1088/2050-6120/2/3/032001

DO - 10.1088/2050-6120/2/3/032001

M3 - Review article

VL - 2

JO - Methods and Applications in Fluorescence

JF - Methods and Applications in Fluorescence

SN - 2050-6120

IS - 3

M1 - 032001

ER -