Experimental validation of a predicted feedback loop in the multi-oscillator clock of Arabidopsis thaliana

James C W Locke, L. Kozma-Bognár, Peter D. Gould, Balázs Fehér, E. Kévei, Ferenc Nagy, Matthew S. Turner, Anthony Hall, Andrew J. Millar

Research output: Contribution to journalArticle

311 Citations (Scopus)

Abstract

Our computational model of the circadian clock comprised the feedback loop between LATE ELONGATED HYPOCOTYL (LHY), CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and TIMING OF CAB EXPRESSION 1 (TOC1), and a predicted, interlocking feedback loop involving TOC1 and a hypothetical component Y. Experiments based on model predictions suggested GIGANTEA (GI) as a candidate for Y. We now extend the model to include a recently demonstrated feedback loop between the TOC1 homologues PSEUDO-RESPONSE REGULATOR 7 (PRR7), PRR9 and LHY and CCA1. This three-loop network explains the rhythmic phenotype of toc1 mutant alleles. Model predictions fit closely to new data on the gi;lhy;cca1 mutant, which confirm that GI is a major contributor to Y function. Analysis of the three-loop network suggests that the plant clock consists of morning and evening oscillators, coupled intracellularly, which may be analogous to coupled, morning and evening clock cells in Drosophila and the mouse.

Original languageEnglish
Article number59
JournalMolecular systems biology [electronic resource].
Volume2
DOIs
Publication statusPublished - May 16 2006

Fingerprint

Arabidopsis Thaliana
Circadian Clocks
Experimental Validation
Feedback Loop
Arabidopsis
Hypocotyl
Clocks
Timing
Arabidopsis thaliana
circadian rhythm
Feedback
Mutant
Prediction Model
hypocotyls
Drosophilidae
Coupled Oscillators
Phenotype
Regulator
Computational Model
mutants

Keywords

  • Circadian rhythm
  • Genetic network
  • Mathematical model
  • Photoperiod
  • Systems biology

ASJC Scopus subject areas

  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Computational Theory and Mathematics
  • Information Systems
  • Applied Mathematics

Cite this

Experimental validation of a predicted feedback loop in the multi-oscillator clock of Arabidopsis thaliana. / Locke, James C W; Kozma-Bognár, L.; Gould, Peter D.; Fehér, Balázs; Kévei, E.; Nagy, Ferenc; Turner, Matthew S.; Hall, Anthony; Millar, Andrew J.

In: Molecular systems biology [electronic resource]., Vol. 2, 59, 16.05.2006.

Research output: Contribution to journalArticle

Locke, James C W ; Kozma-Bognár, L. ; Gould, Peter D. ; Fehér, Balázs ; Kévei, E. ; Nagy, Ferenc ; Turner, Matthew S. ; Hall, Anthony ; Millar, Andrew J. / Experimental validation of a predicted feedback loop in the multi-oscillator clock of Arabidopsis thaliana. In: Molecular systems biology [electronic resource]. 2006 ; Vol. 2.
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