Experimental orthostasis elicits sustained hypertension, which can be prevented by sympathetic blockade in the rat

Gábor Raffai, Márta Mészáros, M. Kollai, E. Monos, László Dézsi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Incidence of orthostatic hypertension is estimated at 5% but is even more prevalent in borderline hypertension and autonomic neuropathies. The aim of this study was to develop a potential model to investigate orthostatic hypertension. We used normotensive and hypertensive Wistar rats to analyze responses and diurnal variations of arterial blood pressure, heart rate, temperature, and locomotor activity by telemetry. Orthostatic tests were carried out during 45° head-up tilt (R, repeated 3 times for 5 minutes; or S, sustained for 120 minutes). Hypertension was induced by blockade of nitric oxide synthesis. In normotensives, horizontal control blood pressure was R115.4 ± 1.4/S113.7 ± 1.6 mm Hg and heart rate R386.4 ± 7.0/S377.9 ± 8.8 bpm. Head-up tilt increased blood pressure by R4.5/S8.4 mm Hg, including a 3.8 mm Hg hydrostatic component. The sustained hypertensive response was prevented by prazosin (10 mg/kgbw) and augmented by a subanesthetic dose of chloralose (26 mg/kgbw). In NO-deprived hypertension, horizontal control blood pressure and heart rate were R138.4 ± 2.6/S140.3 ± 2.7 mm Hg and R342.1 ± 12.0/S346.0 ± 8.3 bpm, respectively. Tilt increased blood pressure further by R4.2/S9.4 mm Hg. In both normo- and hypertensives, variables exhibited similar diurnal rhythms except for nighttime locomotor activity, reduced from 3.7 ± 0.4 to 2.8 ± 0.3 counts/s. These data demonstrate that conscious rats respond to sustained orthostasis with hypertension, probably as a result of increased sympathetic output. Decreasing stress using a subanesthetic dose of chloralose increased this response, reducing the inhibitory effect on hypertensive responses.

Original languageEnglish
Pages (from-to)354-361
Number of pages8
JournalJournal of Cardiovascular Pharmacology
Volume45
Issue number4
DOIs
Publication statusPublished - Apr 2005

Fingerprint

Dizziness
Hypertension
Blood Pressure
Chloralose
Heart Rate
Locomotion
Head
Telemetry
Prazosin
Circadian Rhythm
Wistar Rats
Arterial Pressure
Nitric Oxide
Temperature
Incidence

Keywords

  • α-adrenergic antagonist
  • Anesthetic
  • Autonomic nervous system
  • Conscious rats
  • Endothelial function
  • Orthostatic hypertension

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

Cite this

Experimental orthostasis elicits sustained hypertension, which can be prevented by sympathetic blockade in the rat. / Raffai, Gábor; Mészáros, Márta; Kollai, M.; Monos, E.; Dézsi, László.

In: Journal of Cardiovascular Pharmacology, Vol. 45, No. 4, 04.2005, p. 354-361.

Research output: Contribution to journalArticle

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