Experimental orthostasis elicits sustained hypertension, which can be prevented by sympathetic blockade in the rat

Gábor Raffai, Márta Mészáros, Márk Kollai, Emil Monos, László Dézsi

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10 Citations (Scopus)

Abstract

Incidence of orthostatic hypertension is estimated at 5% but is even more prevalent in borderline hypertension and autonomic neuropathies. The aim of this study was to develop a potential model to investigate orthostatic hypertension. We used normotensive and hypertensive Wistar rats to analyze responses and diurnal variations of arterial blood pressure, heart rate, temperature, and locomotor activity by telemetry. Orthostatic tests were carried out during 45° head-up tilt (R, repeated 3 times for 5 minutes; or S, sustained for 120 minutes). Hypertension was induced by blockade of nitric oxide synthesis. In normotensives, horizontal control blood pressure was R115.4 ± 1.4/S113.7 ± 1.6 mm Hg and heart rate R386.4 ± 7.0/S377.9 ± 8.8 bpm. Head-up tilt increased blood pressure by R4.5/S8.4 mm Hg, including a 3.8 mm Hg hydrostatic component. The sustained hypertensive response was prevented by prazosin (10 mg/kgbw) and augmented by a subanesthetic dose of chloralose (26 mg/kgbw). In NO-deprived hypertension, horizontal control blood pressure and heart rate were R138.4 ± 2.6/S140.3 ± 2.7 mm Hg and R342.1 ± 12.0/S346.0 ± 8.3 bpm, respectively. Tilt increased blood pressure further by R4.2/S9.4 mm Hg. In both normo- and hypertensives, variables exhibited similar diurnal rhythms except for nighttime locomotor activity, reduced from 3.7 ± 0.4 to 2.8 ± 0.3 counts/s. These data demonstrate that conscious rats respond to sustained orthostasis with hypertension, probably as a result of increased sympathetic output. Decreasing stress using a subanesthetic dose of chloralose increased this response, reducing the inhibitory effect on hypertensive responses.

Original languageEnglish
Pages (from-to)354-361
Number of pages8
JournalJournal of cardiovascular pharmacology
Volume45
Issue number4
DOIs
Publication statusPublished - Apr 1 2005

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Keywords

  • Anesthetic
  • Autonomic nervous system
  • Conscious rats
  • Endothelial function
  • Orthostatic hypertension
  • α-adrenergic antagonist

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

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