Sertések fumonizin okozta kísérletes mikotoxikózisa

Translated title of the contribution: Experimental fumonisin mycotoxicosis of swine

Fazekas Béla, Bajmócy Endre, Glávits Róbert, Fenyvesi Adrienne

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Abstract

Fungal culture with a concentration of 7300 mg/kg fumonisin-B1 was prepared with a Fusarium moniliforme strain isolated from corn highly contaminated with fumonisin-B1 mycotoxin according to the method described in the international literature. Fumonisin-B1 content was determined by fluorescent detection after methanol : water = 3:1 mixture extraction, purification on SAX column, forming a derivative with OPA reagent in HPLC. Two piglets weighing approx. 10 and 12 kg were experimentally fed with a feed produced with toxin-free pig rearing nutriment, adding the fungal culture grown on corn up to a ratio obtaining a fumonisin-B1 concentration of 330 mg/kg. Mycotoxin concentration was checked by re-determination. The experimental animals showed faintness even on the first day, their feed was selected by them, however daily 0.5 kg of experimental feed was consumed pro pig. Thus, the daily fumonisin-B1 uptake was 14.5, as well as 16 mg/body-mass kg. Faintness increased by the 3rd to 4th day and both animals died showing severe respiratory symptoms on the 5th day (respiratory rate: 80-100/Min., dyspnoea, abdominal respiration). Approx. 0.5 1 yellowish fluid was found in the thoracic cavity. Both the gross- (Figs 1 and 2) and histopathological examinations (Figs 3 and 4) revealed interlobular and alveolar oedema. Oedema and haemorrhages were found in the heart muscles, while hydropic degeneration and necrosis of parenchymatic cells in the liver (Fig. 5). Small erosion was in the fundal region of stomach, hydropic degeneration in the kidneys, haemorrhages in the adrenal cortex and vacuolar degeneration in the adrenal medulla. Diapedesic haemorrhages, oedema and initial malacia were found in the caudal part of the brain in one of the pigs (Fig. 6). Clinical symptoms, pathological and histological alterations were comparable with those of fumonisin-B1 toxicosis in swine (PPE) observed and experimentally confirmed in the USA between 1989 and 1990. The alterations were also comparable with those of so-called "fattening pulmonary oedema of swine" described by DOMÁN and PETRÁS in Hungary as early as in 1952, that time frequently and nowadays sporadically observed on small farms. The aetiology of this disease has not yet been clarified, however it was generally observed in autumn and was connected to the feeding of the "new" corn. The feeding experiment has confirmed that the so-called "fattening pulmonary oedema" of swine observed for a long time in Hungary, is fumonisin-B1 mycotoxicosis. To prevent fattening oedema of swine, the authors have proposed the determination of the fumonisin-B1 content of corn batches to be used for feeding of swine and avoid the feeding of contaminated batches.

Original languageHungarian
Pages (from-to)10-14
Number of pages5
JournalMagyar Allatorvosok Lapja
Volume119
Issue number1
Publication statusPublished - Dec 1 1997

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ASJC Scopus subject areas

  • veterinary(all)

Cite this

Béla, F., Endre, B., Róbert, G., & Adrienne, F. (1997). Sertések fumonizin okozta kísérletes mikotoxikózisa. Magyar Allatorvosok Lapja, 119(1), 10-14.