A Sertésondó sejtkoncentrációjának mérésére alkalmazott koloriméter kalibrálásával kapcsolatos tapasztalatok

Translated title of the contribution: Experiences with the calibration of colorimeter used for boar semen concetration assessment

Andrés Horváth, Melinda Lénárt, Zsolt Füleki, Lea Lénárt, Zsolt Lang, Ottó Szenci

Research output: Contribution to journalArticle

Abstract

Background: The numeber of artificial insemination (Al)doses which con be obtained from an ejaculate of a single boar have major economic implications in porcine Al centres. This needs a quick and an acceptable precise concentrarin measurements which could be performed with different methods. Objectives: The aim of this study was to compare the accuracy and calibration possibility of a colorimeter -which is a most commonly used method at the Al centre's -to two laboratory concetration assessments as "gold standards" [makler counting chamber and Computer Assisted Sperm Analizer (CASA)]. Materials and Methods: 349 ejaculates warw involved in this study collected from 84 boars. Concentration were measured by means of colorimeter and makler chamber (n=170) or CASA (n=179). Results and Discussion: The recent colorimeter concentration measurement used a linear algorithm which was previously set up with small number of samples (n=15) having narrow absorbances (ABS). This calculation from suggested negative or very low dilution ratio in 5% of the ejaculates but these results were not acceptable for praction. These miscalculations were avoidable if the calibrations were done with large number of samples with brighter ABS but the correlation between concentration and ABS was more exponential then linear by both methods. The colorimeter concentration calibrated by CASA were consistently with 26% higher (p<0.05) then by Makler. Furthermore, the higher were the ABS the differences between concentration calculated by colorimeter and measured By CASA or by Makler became larger. There was a tendency toward over-and underestimation-especially with very high ABS-in concentrations calculated by the colorimeter.

Translated title of the contributionExperiences with the calibration of colorimeter used for boar semen concetration assessment
Original languageHungarian
Pages (from-to)195-201
Number of pages7
JournalMagyar Allatorvosok Lapja
Volume139
Issue number4
Publication statusPublished - Apr 2017

ASJC Scopus subject areas

  • veterinary(all)

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