The objectives of our investigation were to study whether: 1. spindle examination could be included into the clinical use in IVF/ICSI-ET technology, 2. detection of the spindle in MII oocytes with polscope would assist in selecting the best oocytes thus giving more chance for fertilization and development, 3. application of the spindle detection/examination could improve IVF/ICSI-ET results in the clinical use. Following data were compared in the pregnant and non-pregnant group of patients retrospectively: number of 1. obtained oocytes and 2. spindle positive oocytes, and 3. age of the patients. Our observations show that prior to ICSI polar body position indicates the correct spindle position only in 2/3 of the cases, in MII oocytes. Out of 83 IVF/ICSI-ET cycles 38 (38/83; 45.7%) resulted in pregnancy (biochemical: 36%, clinical: 64%). The total number of clinical pregnancies was 24 (24/83; 28.9%). We found differences between the pregnant and non-pregnant group of patients regarding the main number of collected (p<0.02) and spindle positive oocytes (p<0.001). In the pregnant group, the spindle was detected in 68% of the oocytes. However, in the non-pregnant group the spindle was detected in only 54% of the oocytes. Our results indicate that: 1. a higher pregnancy rate can be expected in groups with a higher percentage of spindle positive oocytes, 2. the presence of detectable spindle indicates a better oocyte quality with higher fertilizing and developmental capability, 3. examination of the spindle with polscope can easily be incorporated into the clinical application of IVF/ICSI-ET technology, 4. spindle examination with polscope has no detrimental effects on fertilization, development and implantation of the oocytes, 5. with the increase in patients' age, less number of oocytes and spindle positive oocytes can be obtained thus decreasing the chances of pregnancy.
|Number of pages||5|
|Journal||Magyar Noorvosok Lapja|
|Publication status||Published - Dec 1 2004|
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynaecology