Exogenously applied salicylic acid maintains redox homeostasis in salt-stressed Arabidopsis gr1 mutants expressing cytosolic roGFP1

J. Csiszár, Szilvia Brunner, E. Horváth, Krisztina Bela, Petra Ködmön, Riyazuddin Riyazuddin, Ágnes Gallé, Ágnes Hurton, Csaba Papdi, L. Szabados, I. Tari

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Exogenous salicylic acid (SA) can be used for chemical hardening to alleviate oxidative stress in plants exposed to salinity. The treatment of 5-week-old Arabidopsis thaliana plants with increasing doses of SA alters the ascorbate (ASC) and glutathione (GSH) pools, and modulates their redox status and the activity of several antioxidant enzymes, such as ascorbate peroxidase (APX) and glutathione reductase (GR). To investigate the role of GR in the maintenance of cytoplasmic redox homeostasis after hardening by SA, wild type (WT) and gr1 mutant plants, expressing the cytoplasmic redox-sensitive green fluorescent protein (c-roGFP1), were pre-treated with 10−7 and 10−5 M SA for 2 weeks and subsequently exposed to 100 mM NaCl. The redox status of the salt-stressed WT plants became more oxidized, which was prevented by pretreatment with 10−5 M SA. The gr1 mutants showed more positive redox potential than WT plants, which could be reversed by treatment with 10−5 M SA. In mutants, the increased GSH levels may have compensated for the deleterious effect of GR deficiency and stabilized the redox potential in plants exposed to salinity. The ASC regeneration in WT plants shifted from the GSH-dependent dehydroascorbate reductase (DHAR) reaction to the NAD(P)H-dependent monodehydroascorbate reductase (MDHAR) activity during chemical hardening, which contributed to the preservation of the GSH pool in plants under salt stress. Our results suggest that the maintenance of GSH levels and redox homeostasis by SA-mediated hardening play a major role in priming and defending against salt stress.

Original languageEnglish
Pages (from-to)1-14
Number of pages14
JournalPlant Growth Regulation
DOIs
Publication statusAccepted/In press - Jun 22 2018

Fingerprint

Salicylic Acid
Arabidopsis
salicylic acid
Oxidation-Reduction
homeostasis
Homeostasis
Salts
salts
mutants
Glutathione Reductase
glutathione-disulfide reductase
Salinity
redox potential
glutathione dehydrogenase (ascorbate)
salt stress
Maintenance
Ascorbate Peroxidases
salinity
monodehydroascorbate reductase (NADH)
Green Fluorescent Proteins

Keywords

  • Ascorbate–glutathione pool
  • Glutathione reductase
  • Redox homeostasis
  • Redox-sensitive GFP1
  • Salt stress

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Exogenously applied salicylic acid maintains redox homeostasis in salt-stressed Arabidopsis gr1 mutants expressing cytosolic roGFP1. / Csiszár, J.; Brunner, Szilvia; Horváth, E.; Bela, Krisztina; Ködmön, Petra; Riyazuddin, Riyazuddin; Gallé, Ágnes; Hurton, Ágnes; Papdi, Csaba; Szabados, L.; Tari, I.

In: Plant Growth Regulation, 22.06.2018, p. 1-14.

Research output: Contribution to journalArticle

Csiszár, J. ; Brunner, Szilvia ; Horváth, E. ; Bela, Krisztina ; Ködmön, Petra ; Riyazuddin, Riyazuddin ; Gallé, Ágnes ; Hurton, Ágnes ; Papdi, Csaba ; Szabados, L. ; Tari, I. / Exogenously applied salicylic acid maintains redox homeostasis in salt-stressed Arabidopsis gr1 mutants expressing cytosolic roGFP1. In: Plant Growth Regulation. 2018 ; pp. 1-14.
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abstract = "Exogenous salicylic acid (SA) can be used for chemical hardening to alleviate oxidative stress in plants exposed to salinity. The treatment of 5-week-old Arabidopsis thaliana plants with increasing doses of SA alters the ascorbate (ASC) and glutathione (GSH) pools, and modulates their redox status and the activity of several antioxidant enzymes, such as ascorbate peroxidase (APX) and glutathione reductase (GR). To investigate the role of GR in the maintenance of cytoplasmic redox homeostasis after hardening by SA, wild type (WT) and gr1 mutant plants, expressing the cytoplasmic redox-sensitive green fluorescent protein (c-roGFP1), were pre-treated with 10−7 and 10−5 M SA for 2 weeks and subsequently exposed to 100 mM NaCl. The redox status of the salt-stressed WT plants became more oxidized, which was prevented by pretreatment with 10−5 M SA. The gr1 mutants showed more positive redox potential than WT plants, which could be reversed by treatment with 10−5 M SA. In mutants, the increased GSH levels may have compensated for the deleterious effect of GR deficiency and stabilized the redox potential in plants exposed to salinity. The ASC regeneration in WT plants shifted from the GSH-dependent dehydroascorbate reductase (DHAR) reaction to the NAD(P)H-dependent monodehydroascorbate reductase (MDHAR) activity during chemical hardening, which contributed to the preservation of the GSH pool in plants under salt stress. Our results suggest that the maintenance of GSH levels and redox homeostasis by SA-mediated hardening play a major role in priming and defending against salt stress.",
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AU - Bela, Krisztina

AU - Ködmön, Petra

AU - Riyazuddin, Riyazuddin

AU - Gallé, Ágnes

AU - Hurton, Ágnes

AU - Papdi, Csaba

AU - Szabados, L.

AU - Tari, I.

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