Exo-endocytotic activity during recovery from a brief tetanic stimulation

A role in calcium extrusion?

A. Párducz, F. Loctin, E. Babel-Guérin, Y. Dunant

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Synaptic transmission, metabolism of calcium and ultrastructural changes were investigated at the nerve-electroplaque synapse of Torpedo marmorata during and after a brief tetanic stimulation. Calcium was found to accumulate in stimulated tissue as a function of the number of stimuli; it was subsequently expelled during the recovery period. This period was also accompanied by a marked hydrolysis of energy-rich phosphates (ATP and creatine phosphate). Histochemical localization combined with electron spectroscopic imaging showed calcium deposits in synaptic vesicles and in other substruc- tures. The number of synaptic vesicles containing a calcium deposit transiently increased at the end of activity and declined later during the recovery phase. Rapid cryofixation of the tissue followed by freeze-fracturing revealed membrane openings (pits) in the presynaptic membrane. The density of pits was low in resting tissue; it did not rise during the tetanic stimulation. In contrast, the number of presynaptic pits increased significantly soon after, reaching a maximum value at l min after tetanus. These results are discussed in the light of current hypotheses. They suggest that synaptic vesicles play an important role in intraterminal calcium homeostasis. The vesicles might sequester calcium ions in synaptic terminals during activity and expel them afterwards by exocytosis.

Original languageEnglish
Pages (from-to)93-103
Number of pages11
JournalNeuroscience
Volume62
Issue number1
DOIs
Publication statusPublished - 1994

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Calcium
Synaptic Vesicles
Energy-Filtering Transmission Electron Microscopy
Freeze Fracturing
Torpedo
Membranes
Phosphocreatine
Cryopreservation
Exocytosis
Presynaptic Terminals
Tetanus
Synaptic Transmission
Synapses
Hydrolysis
Homeostasis
Adenosine Triphosphate
Phosphates
Ions

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Exo-endocytotic activity during recovery from a brief tetanic stimulation : A role in calcium extrusion? / Párducz, A.; Loctin, F.; Babel-Guérin, E.; Dunant, Y.

In: Neuroscience, Vol. 62, No. 1, 1994, p. 93-103.

Research output: Contribution to journalArticle

Párducz, A. ; Loctin, F. ; Babel-Guérin, E. ; Dunant, Y. / Exo-endocytotic activity during recovery from a brief tetanic stimulation : A role in calcium extrusion?. In: Neuroscience. 1994 ; Vol. 62, No. 1. pp. 93-103.
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