Exhaled breath condensate in chronic obstructive pulmonary disease: Methodological challenges and clinical application

Zsófia Lázár, I. Horváth, Jørgen Vestbo, András Bikov

Research output: Contribution to journalReview article

Abstract

Collection of exhaled breath condensate (EBC) is a non-invasive method to sample the airway lining fluid. The composition of EBC may reflect physiological and pathophysiological processes within the lower airways, which could otherwise be investigated only with more invasive techniques. Hence, analysis of the condensate fluid seems feasible in chronic obstructive pulmonary disease (COPD) to monitor disease processes and facilitate personalized therapy. In the past two decades, a multitude of molecules has been assessed in EBC samples from patients with COPD, characterizing inflammation, oxidative and nitrative stress in this disorder. Recently, multimarker profiling with sensitive metabolomic or proteomic approaches, optimized for the EBC matrix, has also been applied, which could overcome the pitfalls of single marker detection using commercial assay systems. In this review, we describe the theoretical background of EBC formation, systematically discuss technical and methodological difficulties of sample collection and analysis and summarize data on EBC biomarkers in COPD. Finally, based on previous findings and our experience, we propose potential future directions for the EBC research community, which could pave the way for introducing EBC analysis in clinical practice.

Original languageEnglish
Pages (from-to)42-56
Number of pages15
JournalMinerva Pneumologica
Volume57
Issue number2
DOIs
Publication statusPublished - Jun 1 2018

Fingerprint

Chronic Obstructive Pulmonary Disease
Physiological Phenomena
Metabolomics
Proteomics
Oxidative Stress
Biomarkers
Inflammation
Research
Therapeutics
Direction compound

Keywords

  • Breath tests
  • Chronic obstructive
  • Diagnostic techniques
  • Inflammation mediators
  • Pulmonary disease
  • Respiratory system

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Exhaled breath condensate in chronic obstructive pulmonary disease : Methodological challenges and clinical application. / Lázár, Zsófia; Horváth, I.; Vestbo, Jørgen; Bikov, András.

In: Minerva Pneumologica, Vol. 57, No. 2, 01.06.2018, p. 42-56.

Research output: Contribution to journalReview article

@article{390c6ec9949d4c6e97fab4770b1e7db2,
title = "Exhaled breath condensate in chronic obstructive pulmonary disease: Methodological challenges and clinical application",
abstract = "Collection of exhaled breath condensate (EBC) is a non-invasive method to sample the airway lining fluid. The composition of EBC may reflect physiological and pathophysiological processes within the lower airways, which could otherwise be investigated only with more invasive techniques. Hence, analysis of the condensate fluid seems feasible in chronic obstructive pulmonary disease (COPD) to monitor disease processes and facilitate personalized therapy. In the past two decades, a multitude of molecules has been assessed in EBC samples from patients with COPD, characterizing inflammation, oxidative and nitrative stress in this disorder. Recently, multimarker profiling with sensitive metabolomic or proteomic approaches, optimized for the EBC matrix, has also been applied, which could overcome the pitfalls of single marker detection using commercial assay systems. In this review, we describe the theoretical background of EBC formation, systematically discuss technical and methodological difficulties of sample collection and analysis and summarize data on EBC biomarkers in COPD. Finally, based on previous findings and our experience, we propose potential future directions for the EBC research community, which could pave the way for introducing EBC analysis in clinical practice.",
keywords = "Breath tests, Chronic obstructive, Diagnostic techniques, Inflammation mediators, Pulmonary disease, Respiratory system",
author = "Zs{\'o}fia L{\'a}z{\'a}r and I. Horv{\'a}th and J{\o}rgen Vestbo and Andr{\'a}s Bikov",
year = "2018",
month = "6",
day = "1",
doi = "10.23736/S0026-4954.18.01816-3",
language = "English",
volume = "57",
pages = "42--56",
journal = "Minerva Pneumologica",
issn = "0026-4954",
publisher = "Edizioni Minerva Medica S.p.A.",
number = "2",

}

TY - JOUR

T1 - Exhaled breath condensate in chronic obstructive pulmonary disease

T2 - Methodological challenges and clinical application

AU - Lázár, Zsófia

AU - Horváth, I.

AU - Vestbo, Jørgen

AU - Bikov, András

PY - 2018/6/1

Y1 - 2018/6/1

N2 - Collection of exhaled breath condensate (EBC) is a non-invasive method to sample the airway lining fluid. The composition of EBC may reflect physiological and pathophysiological processes within the lower airways, which could otherwise be investigated only with more invasive techniques. Hence, analysis of the condensate fluid seems feasible in chronic obstructive pulmonary disease (COPD) to monitor disease processes and facilitate personalized therapy. In the past two decades, a multitude of molecules has been assessed in EBC samples from patients with COPD, characterizing inflammation, oxidative and nitrative stress in this disorder. Recently, multimarker profiling with sensitive metabolomic or proteomic approaches, optimized for the EBC matrix, has also been applied, which could overcome the pitfalls of single marker detection using commercial assay systems. In this review, we describe the theoretical background of EBC formation, systematically discuss technical and methodological difficulties of sample collection and analysis and summarize data on EBC biomarkers in COPD. Finally, based on previous findings and our experience, we propose potential future directions for the EBC research community, which could pave the way for introducing EBC analysis in clinical practice.

AB - Collection of exhaled breath condensate (EBC) is a non-invasive method to sample the airway lining fluid. The composition of EBC may reflect physiological and pathophysiological processes within the lower airways, which could otherwise be investigated only with more invasive techniques. Hence, analysis of the condensate fluid seems feasible in chronic obstructive pulmonary disease (COPD) to monitor disease processes and facilitate personalized therapy. In the past two decades, a multitude of molecules has been assessed in EBC samples from patients with COPD, characterizing inflammation, oxidative and nitrative stress in this disorder. Recently, multimarker profiling with sensitive metabolomic or proteomic approaches, optimized for the EBC matrix, has also been applied, which could overcome the pitfalls of single marker detection using commercial assay systems. In this review, we describe the theoretical background of EBC formation, systematically discuss technical and methodological difficulties of sample collection and analysis and summarize data on EBC biomarkers in COPD. Finally, based on previous findings and our experience, we propose potential future directions for the EBC research community, which could pave the way for introducing EBC analysis in clinical practice.

KW - Breath tests

KW - Chronic obstructive

KW - Diagnostic techniques

KW - Inflammation mediators

KW - Pulmonary disease

KW - Respiratory system

UR - http://www.scopus.com/inward/record.url?scp=85048714005&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85048714005&partnerID=8YFLogxK

U2 - 10.23736/S0026-4954.18.01816-3

DO - 10.23736/S0026-4954.18.01816-3

M3 - Review article

AN - SCOPUS:85048714005

VL - 57

SP - 42

EP - 56

JO - Minerva Pneumologica

JF - Minerva Pneumologica

SN - 0026-4954

IS - 2

ER -