Excretion of primary prostanoids and their metabolites during acute volume expansion

Hannsjörg W. Seyberth, Tivadar Tulassay, P. Gonne Kühl, Karin Soeding, Wolfgang Rascher, Horst Schweer

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Abstract

Simultaneous determination of urinary excretion rates of primary unmetabolized prostanoids and their enzymatic metabolites were performed by gas chromatography-mass spectrometry (GC/MS) or tandem mass spectrometry (GC/MS/MS). Changes in kidney function were induced by acute (4 h) volume expansion. Despite marked changes in urine flow, GFR, urinary pH, osmolality, sodium and potassium excretion, only a insignificant or transient rise in the enzymatic prostanoid metabolites (2,3-dinor-6-keto-PGF, PGE-M, 2,3-dinor-TxB2 and 11-dehydro-TxB2) was observed. The excretion rates of the primary prostanoids were elevated in parallel with the rise in urine flow: PGE2 rose (p < 0.05) from 14.2 ± 4.0 to 86.2 ± 20.7, PGF from 60.0 ± 4.9 to 119.8 ± 24.0, 6-keto-PGF from 7.2 ± 1.3 to 51.5 ± 17.0, and txB2 from 11.2 ± 3.3 to 13.6 ± 3.6 ng/h/1.73 m2 ( x ̄ ± SEM) at the maximal urine flow. Except for 6-keto-PGF and TxB2, this rise in urinary prostanoid levels was only transient despite a sustained fourfold elevated urine flow. We conclude that urine flow rate acutely affect urine prostanoid excretion rates, however, over a prolonged peroid of time these effects are not maintained. The present data support the concept that urinary levels of primary prostanoids mainly reflect renal concentrations whereas those of enzymatic metabolites reflect systemic prostanoid activity. From the excretion pattern of TxB2 one can assume that this prostanoid represents renal as well as systemic TxA2 activity.

Original languageEnglish
Pages (from-to)221-232
Number of pages12
JournalProstaglandins
Volume35
Issue number2
DOIs
Publication statusPublished - Feb 1988

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ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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