Excitation of the M intermediates of bacteriorhodopsin

Rudolf Tóth-Boconádi, A. Dér, László Fábián, Stefka Germanova Taneva, Lajos Keszthelyi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Protein electric response signals (PERS) of the M intermediates of wild-type bacteriorhodopsin (bR) were recorded. Contrary to earlier findings reporting on a single-phase response upon excitation of the M intermediates, a kinetic analysis of the signals revealed the existence of three components, the fastest and the slowest ones of negative, while the middle one of positive sign with respect to the normal direction of proton pumping. Based on proton motion indicator experiments and molecular dipole calculations, the components were assigned to proton transfer steps and conformational changes driving the bR molecule back from the M to the ground state upon blue light excitation. The fastest, negative pump component was assigned to the proton transfer from D85 to the Schiff base. The subsequent positive component was attributed to rearrangements in the protein core (in the vicinity of the retinal molecule), triggered by the primary proton transfer process. The slowest component was established to reflect charge rearrangements associated with proton uptake by the protein from the bulk.

Original languageEnglish
Pages (from-to)609-613
Number of pages5
JournalPhotochemistry and Photobiology
Volume85
Issue number2
DOIs
Publication statusPublished - Mar 2009

Fingerprint

Bacteriorhodopsins
Proton transfer
Protons
protons
excitation
Proteins
Molecules
Schiff Bases
proteins
Ground state
Pumps
phase response
Kinetics
imines
molecules
pumping
Experiments
pumps
dipoles
Light

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Biochemistry
  • Medicine(all)

Cite this

Excitation of the M intermediates of bacteriorhodopsin. / Tóth-Boconádi, Rudolf; Dér, A.; Fábián, László; Taneva, Stefka Germanova; Keszthelyi, Lajos.

In: Photochemistry and Photobiology, Vol. 85, No. 2, 03.2009, p. 609-613.

Research output: Contribution to journalArticle

Tóth-Boconádi, R, Dér, A, Fábián, L, Taneva, SG & Keszthelyi, L 2009, 'Excitation of the M intermediates of bacteriorhodopsin', Photochemistry and Photobiology, vol. 85, no. 2, pp. 609-613. https://doi.org/10.1111/j.1751-1097.2008.00521.x
Tóth-Boconádi, Rudolf ; Dér, A. ; Fábián, László ; Taneva, Stefka Germanova ; Keszthelyi, Lajos. / Excitation of the M intermediates of bacteriorhodopsin. In: Photochemistry and Photobiology. 2009 ; Vol. 85, No. 2. pp. 609-613.
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