Excimer laser forward transfer of mammalian cells using a novel triazene absorbing layer

A. Doraiswamy, R. J. Narayan, T. Lippert, L. Urech, A. Wokaun, M. Nagel, B. Hopp, M. Dinescu, R. Modi, R. C Y Auyeung, D. B. Chrisey

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

We present a novel laser-based approach for developing tissue engineered constructs and other cell-based assembly's. We have deposited mesoscopic patterns of viable B35 neuroblasts using a soft direct approach of the matrix assisted pulsed laser evaporation direct write (MAPLE DW) process. As a development of the conventional direct write process, an intermediate layer of absorbing triazene polymer is used to provide gentler and efficient transfers. Transferred cells were examined for viability and proliferation and compared with that of as-seeded cells to determine the efficacy of the process. Results suggest that successful transfers can be achieved at lower fluences than usual by the incorporation of the intermediate absorbing layer thus avoiding any damage to cells and other delicate materials. MAPLE DW offers rapid computer-controlled deposition of mesoscopic voxels at high spatial resolutions, with extreme versatility in depositing combinations of natural/synthetic, living/non-living, organic/inorganic and hard/soft materials. Our approach offers a gentle and efficient transfer of viable cells which when combined with a variety of matrix materials allows development of constructs and bioactive systems in bioengineering.

Original languageEnglish
Pages (from-to)4743-4747
Number of pages5
JournalApplied Surface Science
Volume252
Issue number13 SPEC. ISS.
DOIs
Publication statusPublished - Apr 30 2006

Fingerprint

Triazenes
Excimer lasers
excimer lasers
Cells
Pulsed lasers
Evaporation
cells
neuroblasts
pulsed lasers
evaporation
bioengineering
Polymers
matrix materials
Tissue
versatility
matrices
activity (biology)
viability
Lasers
fluence

Keywords

  • Laser forward transfer
  • MAPLE DW
  • Triazene polymer

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Condensed Matter Physics

Cite this

Doraiswamy, A., Narayan, R. J., Lippert, T., Urech, L., Wokaun, A., Nagel, M., ... Chrisey, D. B. (2006). Excimer laser forward transfer of mammalian cells using a novel triazene absorbing layer. Applied Surface Science, 252(13 SPEC. ISS.), 4743-4747. https://doi.org/10.1016/j.apsusc.2005.07.166

Excimer laser forward transfer of mammalian cells using a novel triazene absorbing layer. / Doraiswamy, A.; Narayan, R. J.; Lippert, T.; Urech, L.; Wokaun, A.; Nagel, M.; Hopp, B.; Dinescu, M.; Modi, R.; Auyeung, R. C Y; Chrisey, D. B.

In: Applied Surface Science, Vol. 252, No. 13 SPEC. ISS., 30.04.2006, p. 4743-4747.

Research output: Contribution to journalArticle

Doraiswamy, A, Narayan, RJ, Lippert, T, Urech, L, Wokaun, A, Nagel, M, Hopp, B, Dinescu, M, Modi, R, Auyeung, RCY & Chrisey, DB 2006, 'Excimer laser forward transfer of mammalian cells using a novel triazene absorbing layer', Applied Surface Science, vol. 252, no. 13 SPEC. ISS., pp. 4743-4747. https://doi.org/10.1016/j.apsusc.2005.07.166
Doraiswamy A, Narayan RJ, Lippert T, Urech L, Wokaun A, Nagel M et al. Excimer laser forward transfer of mammalian cells using a novel triazene absorbing layer. Applied Surface Science. 2006 Apr 30;252(13 SPEC. ISS.):4743-4747. https://doi.org/10.1016/j.apsusc.2005.07.166
Doraiswamy, A. ; Narayan, R. J. ; Lippert, T. ; Urech, L. ; Wokaun, A. ; Nagel, M. ; Hopp, B. ; Dinescu, M. ; Modi, R. ; Auyeung, R. C Y ; Chrisey, D. B. / Excimer laser forward transfer of mammalian cells using a novel triazene absorbing layer. In: Applied Surface Science. 2006 ; Vol. 252, No. 13 SPEC. ISS. pp. 4743-4747.
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