Examinations of allergenic proteins coding genes of some domestic apple cultivars

E. Szabó, E. Gelencsér, E. Kovács, A. Jánosi, K. Takács, E. Kiss

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In our research we studied the occurrence of the main apple allergen coding gene-families (Mal d 1, Mal d 2, Mal d 3, Mal d 4) in 16 different and most preferably consumed apple varieties. After the DNA isolation by Wizard method the simple PCR reaction was used to examine the apple allergen-coding genes. To identify the presence of the four allergenic protein-coding genes two primer pairs were chosen. The presence of these allergens in most apple varieties could be confirmed. According to our results two varieties-Jonathan and Granny Smith-were found to contain the lowest amount of the coding genes of the allergenic apple proteins studied by us. Besides this, polymorph pattern was obtained by the use of Mal d 1 primer, which may lead to determine apple varieties with small amount of Mal d 1 allergens.The confirmation study of the presence of potential apple allergens by RNA and protein techniques is our plan in the near future.

Original languageEnglish
Pages (from-to)8-18
Number of pages11
JournalActa Alimentaria
Volume41
Issue number1
DOIs
Publication statusPublished - Mar 1 2012

    Fingerprint

Keywords

  • Mal d allergenswhom
  • PCR method
  • apple allergy

ASJC Scopus subject areas

  • Food Science

Cite this