Examination of typical bacterial strains in septic arthritis by isoperibol calorimeter

Dandé, L. G. Nőt, G. Bűcs, N. Wiegand, B. Kocsis, D. Lőrinczy

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The accurate diagnosis of different types of arthritis is a great clinical challenge. It has a special importance to reach the right diagnosis in case of septic joint infections. The clarification of aetiology is crucial to minimize the complications and mortality of these cases. Therefore, novel methods like synovial sample analyses by differential scanning calorimetry (DSC) could have the potential to provide further information in the diagnostic process (Dandé et al. in J Therm Anal Calorim. doi:https://doi.org/10.1007/s10973-017-6179-6, 2017). A retrospective analysis of patients treated with septic joint infections over the last 5 years at the Department of Traumatology and Hand Surgery has been conducted. Human synovial samples were inoculated with six different, frequently occurring human pathogenic bacterial strains. The proliferation of inoculated synovial fluid samples was monitored by SETARAM Micro DSC-II calorimeter in isotherm mode (isoperibol calorimeter). Based on our results, 47.45% of the cases involved small joint (SG) infections and 52.55% of them occurred in large joints (LG). Synovial aspirates collected from infected joints indicated the presence of the following bacteria and incidences: Staphylococcus aureus 43 (22.16%), Staphylococcus epidermidis 3 (1.54%), Methicillin-resistant Staphylococcus aureus, MRSA 4 (2.06%), Streptococcus pyogenes 9 (4.63%), Escherichia coli 1 (0.51%) and Pseudomonas aeruginosa 4 (2.06%). The isoperibolic curves of human synovial samples inoculated with different bacterial strains clearly demonstrated specific/representative results in case of each bacterium. The control of human synovial fluid samples by isotherm calorimetry could be a useful tool in the differential diagnosis of human septic arthritis.

Original languageEnglish
Pages (from-to)2041-2048
Number of pages8
JournalJournal of Thermal Analysis and Calorimetry
Volume131
Issue number3
DOIs
Publication statusPublished - Mar 1 2018

Fingerprint

arthritis
Calorimeters
calorimeters
examination
staphylococcus
infectious diseases
Isotherms
Differential scanning calorimetry
Bacteria
heat measurement
Methicillin
Fluids
bacteria
Calorimetry
isotherms
Surgery
Escherichia coli
streptococcus
etiology
pseudomonas

Keywords

  • Bacterial strains
  • Isoperibolic calorimetry
  • Septic arthritis
  • Synovial fluid

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

Cite this

Examination of typical bacterial strains in septic arthritis by isoperibol calorimeter. / Dandé; Nőt, L. G.; Bűcs, G.; Wiegand, N.; Kocsis, B.; Lőrinczy, D.

In: Journal of Thermal Analysis and Calorimetry, Vol. 131, No. 3, 01.03.2018, p. 2041-2048.

Research output: Contribution to journalArticle

Dandé ; Nőt, L. G. ; Bűcs, G. ; Wiegand, N. ; Kocsis, B. ; Lőrinczy, D. / Examination of typical bacterial strains in septic arthritis by isoperibol calorimeter. In: Journal of Thermal Analysis and Calorimetry. 2018 ; Vol. 131, No. 3. pp. 2041-2048.
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