Examination of the peripheral nerve and muscle damage in cyclophosphamide monotherapy with DSC in animal models

Péter Farkas, Franciska Könczöl, D. Lőrinczy

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Polyneuropathy is defined as a simultaneous malfunction of several peripheral nerves, which could be a side effect of cancer therapy; however, this is reported to occur rarely and difficult to prove. The purpose of this study was to introduce at the first time the calorimetry in the diagnosis of neuropathy in an experimental animal model. Adult guinea pigs (n = 55, in 11 different groups) were injected intraperitoneally with the dose of cyclophosphamide that is comparable to the human dosage. Animals were euthanized; nerve (ischiadicus) and muscle (gastrocnemius) samples were analyzed by a SETARAM Micro-DSC. The denaturation temperatures as well as the calorimetric enthalpies were determined. The thermal parameters of denaturation of the samples varied depending on the therapeutic cyclophosphamide doses. The nerves were more sensitive to chemotherapy, compared to the muscles. An interesting body side effect of treatment was observed. The DSC is an appropriate tool to follow the effects of cyclophosphamide on peripheral nerves and muscles, which were found dose dependent.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of Thermal Analysis and Calorimetry
DOIs
Publication statusAccepted/In press - Jan 30 2016

Fingerprint

animal models
nerves
muscles
Cyclophosphamide
Muscle
Animals
Denaturation
examination
damage
dosage
biopolymer denaturation
Chemotherapy
Calorimetry
guinea pigs
malfunctions
Enthalpy
chemotherapy
animals
therapy
heat measurement

Keywords

  • Blood cell and plasma
  • Cyclophosphamide
  • DSC
  • m. gastrocnemius
  • Polyneuropathia
  • Sciatic nerve

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Condensed Matter Physics

Cite this

Examination of the peripheral nerve and muscle damage in cyclophosphamide monotherapy with DSC in animal models. / Farkas, Péter; Könczöl, Franciska; Lőrinczy, D.

In: Journal of Thermal Analysis and Calorimetry, 30.01.2016, p. 1-7.

Research output: Contribution to journalArticle

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