Examination of the growth of probiotic culture combinations by the isoperibolic batch calorimetry

B. Schäffer, S. Szakály, D. Lorinczy

Research output: Contribution to journalArticle

5 Citations (Scopus)


Probiotic ("intestinal friend") foods, and probiotic dairy products in particular, have substantially increased in number recently. In the production of some of these products a problem is caused by the fact that the thermotolerant microbes that produce the probiotic effect do not generate an aroma; the taste of the product is given by aroma-producing mesophilic microbes. The growth optima of the two microbe groups do not coincide. Given that heat is released in the course of microbe reproduction, the isoperibolic calorimetry method appears the best and fastest for monitoring the process. During the experiments the thermotolerant Prebiolact, owned by the Hungarian Dairy Research Institute (HDRI) and clinically verified to be probiotic, and Hansen Company's CHN-22 mesophilic butter culture were examined bred separately and together. The cultures were incubated at 30°C, at which temperature prior studies had shown both lactic acid bacteria cultures were capable of growing. The cultures were injected into milk, then the microbe growth heat flow-time curve over 18 h was taken on the Setaram Micro DSC-II calorimeter at 30°C under isothermic conditions. Analysis of the heat flow curves led to the following conclusions. Both lactic acid bacteria cultures grow well at the non-optimal temperature of 30°C, thermotolerant Prebiolact somewhat faster and mesophilic CHN-22 slower. The two cultures do not impede each other in mixed cultures; the growth peaks of the two cultures were easily isolated on the power-time curve by a deconvolution program. In sum it can be stated that mixed cultures of the two cultures examined can be used to produce dairy products which are probiotic, but their taste character (e.g. aroma) is determined by the butter culture. To date we have elaborated production procedures for probiotic butter cream and heat-resistant sour cream using the two cultures in a mixed one.

Original languageEnglish
Pages (from-to)123-126
Number of pages4
JournalThermochimica Acta
Issue number1-2
Publication statusPublished - Jun 7 2004


  • Isoperibolic batch calorimetry
  • Microbial growth
  • Ratio of probiotic and aromatic microbes

ASJC Scopus subject areas

  • Instrumentation
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

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