Examination of the effect of sodium nitrite on gap junction function during ischaemia and reperfusion in anaesthetized dogs

Gottfried Miskolczi, Márton Gönczi, Mária Kovács, A. Végh

Research output: Contribution to journalArticle

Abstract

It has previously been proved that sodium nitrite, infused prior to coronary artery occlusion or before reperfusion, results in marked antiarrhythmic effect in anaesthetized dogs. We have now examined whether this protection involves the modulation of gap junction (GJ) function by nitric oxide (NO), derived from nitrite administration under ischaemic conditions. Two groups of chloralose and urethane anaesthetized dogs, each containing 13 animals, were subjected to a 25 min period occlusion of the left anterior descending (LAD) coronary artery, followed by reperfusion. One group was infused with sodium nitrite (0.2 μmol/kg/min, i.v.), the other group with saline 10 min prior to reperfusion. The severities of arrhythmias and of ischaemia (epicardial ST-segment, total activation time), parallel with changes in myocardial tissue impedance, a measure of electrical coupling of gap junctions, were assessed during the experiments. Compared to the controls, nitrite infusion administered prior to reperfusion significantly attenuated the severity of ischaemia, the ischaemia-induced impedance changes and, consequently, the severity of arrhythmias, occurring during the 1B phase of the occlusion, and increase survival following reperfusion (0% vs. 85%). It is concluded that the marked antiarrhythmic effect of sodium nitrite is partly due, to the preservation of the electrical coupling of GJs by NO.

Original languageEnglish
Pages (from-to)35-49
Number of pages15
JournalActa Biologica Hungarica
Volume68
Issue number1
DOIs
Publication statusPublished - Mar 1 2017

Fingerprint

Sodium Nitrite
Gap Junctions
nitrite
Reperfusion
Ischemia
sodium
Dogs
Nitrites
Nitric Oxide
nitric oxide
Chloralose
Electric Impedance
Urethane
Cardiac Arrhythmias
Coronary Vessels
Animals
Chemical activation
Modulation
Tissue
Coronary Occlusion

Keywords

  • Arrhythmia
  • Gap junction
  • Ischaemia/reperfusion
  • Myocardial tissue impedance
  • Sodium nitrite

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Cite this

Examination of the effect of sodium nitrite on gap junction function during ischaemia and reperfusion in anaesthetized dogs. / Miskolczi, Gottfried; Gönczi, Márton; Kovács, Mária; Végh, A.

In: Acta Biologica Hungarica, Vol. 68, No. 1, 01.03.2017, p. 35-49.

Research output: Contribution to journalArticle

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