The aim of the study was to evaluate the effect of negative energy balance manifesting in elevated plasma NEFA concentration on the anatomical involution of the uterus, on the reactivation of ovarian function, and on the incidence of retained placenta and metritis. Clinically healthy Holstein-Friesian dairy cows were involved in the survey, which started 10 days before their expected calvings. The energy metabolism of the cows was evaluated by determining the body condition score (BCS), the plasma concentrations of non-esterified fatty acids (NEFA), acetoacetic acid (AcA), glucose and aspartat- aminotransferase (AST) activity. Blood samples were taken on day 10 prepartum, on the 3 rd day postpartum (pp.) and on every 10th day onwards until day 60 postpartum. Onset of cyclic ovarian activity was monitored by analysing the plasma progesterone (P4) concentration on every 3 rd day from days 20 to 21 until days 59 to 60 postpartum. The uterus was clinically examined by palpation on days 5, 20 and 40, respectively. Transrectal ultrasonography was performed on days 30 and 60 to check the uterine involution and to follow up the structural changes of the ovaries. Data were collected on the incidence of retained placenta and postpartum uterine diseases, and the onset of oestrus. Cows were divided into two groups on the basis of their plasma NEFA concentration. Cows with 0.2 mmol/l or higher plasma NEFA concentration from at least two subsequent samplings formed one of the groups (H-NEFA group, n=12). The other group (normal group, n=16) had lower plasma NEFA concentration than the aforementioned threshold. In the H-NEFA group, on days 10 and 20 after calving the AST activity (P<0.05), on day 10 the plasma AcA concentration (P>0.05), the frequency of the incidence of subclinical ketosis and precalving BCS (P<0.05) were higher than the respective values in the normal group. Plasma glucose concentration of the H-NEFA cows on day 3 was lower (P>0.05) than that of the normal cows. In the H-NEFA group, uterine score was higher (2.8 vs. 2.2; P<0.05) on day 30 pp. Corpus luteum was less frequently present in H-NEFA cows until days 30 and 60 pp. (8.3% vs. 18.8% and 33.3% vs. 75.0%, respectively). The first P4 phase were detected in lower percentage of H-NEFA cows until days 28 (8.3% vs. 31.3%) and 35 (25.0% vs. 43.8%) postpartum. Proportion of oestrus cows was less in the H-NEFA group until day 60 pp. (6.3% vs. 25.0%). Until day 30 pp. the incidence of retained placenta was higher in the H-NEFA cows (41.7% vs. 18.8%). The proportion of clinically diagnosed postpartum uterine disease was also higher in the H-NEFA cows (until days 30 and 60 pp.: 100.0% vs. 68.8% and 66.7% vs. 37.5%, respectively). Percentage of oestrus cows in the first 60 days pp. was 6.3% in H- NEFA group and 25.0% in the normal group.
|Number of pages||11|
|Journal||Magyar Allatorvosok Lapja|
|Publication status||Published - Jun 22 2009|
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