A PACAP-38 citoprotektív és antiinflammatórikus hatásának vizsgálata vékonybél-autotranszplantációs modellben

Translated title of the contribution: Examination of cytoprotective and anti-inflammatory effect of PACAP-38 on small bowel autotransplantation

Klára Nedvig, Györgyi Szabó, Domokos Csukás, József Sándor, József Németh, Krisztina Kovács, Dóra Reglődi, Agnes Kemény, György Wéber, Andrea Ferencz

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

INTRODUCTION: The small intestine is one of the most sensitive organs to ischemia-reperfusion injury during transplantation. Cytoprotective effect of pituitary adenylate cyclase-activating polypeptide (PACAP) is well known. The aim of our study was to measure changes of PACAP-38-like immunoreactivities and cytokine levels in intestinal grafts stored PACAP-38 containing preservation solution.

MATERIAL AND METHODS: Small-bowel autotransplantation was performed on male Wistar rats (n = 56). Grafts were stored in University of Wisconsin (UW) solution at 4 °C for 1 (GI), 3 (GII), and 6 hours (GIII); and in PACAP-38 containing UW solution for 1 (GIV), 3 (GV), and 6 hours (GVI). Reperfusion lasted 3 hours in each group. Intestinal PACAP-38 immunoreactivities were measured by radioimmunoassay. To measure cytokine from tissue homogenates we used rat cytokine array and Luminex Multiplex Immunoassay.

RESULTS: Levels of PACAP-38-like and PACAP-27-like immunoreactivities decreased by preservation time compared to control. This decrease was significant following 6 hours cold storage (p < 0.05). Values remained significantly higher in grafts stored in PACAP-38 containing UW. Expressions of sICAM-1, L-selectin, tissue inhibitor of metalloproteinase-1 were increased in GIII and were decreased in GVI.

CONCLUSION: PACAP-38 increased tissue levels of PACAP-38 and PACAP-27, and decreased cytokine expression. This indicates that PACAP-38 has anti-inflammatory and cytoprotective effects in intestinal autotransplantation model.

Translated title of the contributionExamination of cytoprotective and anti-inflammatory effect of PACAP-38 on small bowel autotransplantation
Original languageHungarian
Pages (from-to)250-255
Number of pages6
JournalMagyar sebészet
Volume66
Issue number5
DOIs
Publication statusPublished - Oct 1 2013

ASJC Scopus subject areas

  • Medicine(all)

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