Examination of cfiA-mediated carbapenem resistance in Bacteroides fragilis strains from a European antibiotic susceptibility survey

J. Sóki, R. Edwards, M. Hedberg, H. Fang, E. Nagy, C. E. Nord

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Of 1284 Bacteroides strains collected in Europe in 2000 for antibiotic susceptibility surveillance, 65 isolates displayed imipenem minimum inhibitory concentrations (MICs) ≥1 mg/L and were chosen for a thorough analysis of their resistance mechanism. Twenty-five of the isolates were positive for the cfiA carbapenem resistance gene. The resistance rates were 0.8% and 1.3% for imipenem and meropenem, respectively. In six of the strains, insertion sequence (IS) elements (IS613, IS614B, IS1186 and IS1187) activated the cfiA gene. However, other strains displayed at least elevated carbapenem MICs or were carbapenem resistant and produced measurable carbapenemase activities but did not harbour IS elements in the region upstream of the cfiA gene. The major determinant of carbapenem resistance in Bacteroides fragilis is production of CfiA metallo-β-lactamase via activation of the cfiA gene by IS elements (higher level resistance) or by activation of its putative own promoter.

Original languageEnglish
Pages (from-to)497-502
Number of pages6
JournalInternational Journal of Antimicrobial Agents
Volume28
Issue number6
DOIs
Publication statusPublished - Dec 1 2006

Keywords

  • Bacteroides fragilis
  • Imipenem
  • Insertion sequence (IS) elements
  • Meropenem
  • cfiA

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

Fingerprint Dive into the research topics of 'Examination of cfiA-mediated carbapenem resistance in Bacteroides fragilis strains from a European antibiotic susceptibility survey'. Together they form a unique fingerprint.

  • Cite this