Ex vivo nonlinear microscopy imaging of Ehlers–Danlos syndrome-affected skin

Norbert Kiss, Dóra Haluszka, Kende Lőrincz, Enikő Kuroli, Judit Hársing, B. Mayer, S. Kárpáti, G. Fekete, R. Szipócs, N. Wikonkál, Márta Medvecz

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Ehlers–Danlos syndrome (EDS) is the name for a heterogenous group of rare genetic connective tissue disorders with an overall incidence of 1 in 5000. The histological characteristics of EDS have been previously described in detail in the late 1970s and early 1980s. Since that time, the classification of EDS has undergone significant changes, yet the description of the histological features of collagen morphology in different EDS subtypes has endured the test of time. Nonlinear microscopy techniques can be utilized for non-invasive in vivo label-free imaging of the skin. Among these techniques, two-photon absorption fluorescence (TPF) microscopy can visualize endogenous fluorophores, such as elastin, while the morphology of collagen fibers can be assessed by second-harmonic generation (SHG) microscopy. In our present work, we performed TPF and SHG microscopy imaging on ex vivo skin samples of one patient with classical EDS and two patients with vascular EDS and two healthy controls. We detected irregular, loosely dispersed collagen fibers in a non-parallel arrangement in the dermis of the EDS patients, while as expected, there was no noticeable impairment in the elastin content. Based on further studies on a larger number of patients, in vivo nonlinear microscopic imaging could be utilized for the assessment of the skin status of EDS patients in the future.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalArchives of Dermatological Research
DOIs
Publication statusAccepted/In press - May 3 2018

Fingerprint

Microscopy
Skin
Collagen
Elastin
Photons
Dermis
Fluorescence Microscopy
Connective Tissue
Names
Blood Vessels
Fluorescence
Incidence

Keywords

  • Collagen structure
  • Ehlers–Danlos syndrome
  • Nonlinear microscopy
  • Second-harmonic generation

ASJC Scopus subject areas

  • Dermatology

Cite this

Kiss, N., Haluszka, D., Lőrincz, K., Kuroli, E., Hársing, J., Mayer, B., ... Medvecz, M. (Accepted/In press). Ex vivo nonlinear microscopy imaging of Ehlers–Danlos syndrome-affected skin. Archives of Dermatological Research, 1-11. https://doi.org/10.1007/s00403-018-1835-7

Ex vivo nonlinear microscopy imaging of Ehlers–Danlos syndrome-affected skin. / Kiss, Norbert; Haluszka, Dóra; Lőrincz, Kende; Kuroli, Enikő; Hársing, Judit; Mayer, B.; Kárpáti, S.; Fekete, G.; Szipócs, R.; Wikonkál, N.; Medvecz, Márta.

In: Archives of Dermatological Research, 03.05.2018, p. 1-11.

Research output: Contribution to journalArticle

Kiss, Norbert ; Haluszka, Dóra ; Lőrincz, Kende ; Kuroli, Enikő ; Hársing, Judit ; Mayer, B. ; Kárpáti, S. ; Fekete, G. ; Szipócs, R. ; Wikonkál, N. ; Medvecz, Márta. / Ex vivo nonlinear microscopy imaging of Ehlers–Danlos syndrome-affected skin. In: Archives of Dermatological Research. 2018 ; pp. 1-11.
@article{072998938da94da3adc97197e7e40a5d,
title = "Ex vivo nonlinear microscopy imaging of Ehlers–Danlos syndrome-affected skin",
abstract = "Ehlers–Danlos syndrome (EDS) is the name for a heterogenous group of rare genetic connective tissue disorders with an overall incidence of 1 in 5000. The histological characteristics of EDS have been previously described in detail in the late 1970s and early 1980s. Since that time, the classification of EDS has undergone significant changes, yet the description of the histological features of collagen morphology in different EDS subtypes has endured the test of time. Nonlinear microscopy techniques can be utilized for non-invasive in vivo label-free imaging of the skin. Among these techniques, two-photon absorption fluorescence (TPF) microscopy can visualize endogenous fluorophores, such as elastin, while the morphology of collagen fibers can be assessed by second-harmonic generation (SHG) microscopy. In our present work, we performed TPF and SHG microscopy imaging on ex vivo skin samples of one patient with classical EDS and two patients with vascular EDS and two healthy controls. We detected irregular, loosely dispersed collagen fibers in a non-parallel arrangement in the dermis of the EDS patients, while as expected, there was no noticeable impairment in the elastin content. Based on further studies on a larger number of patients, in vivo nonlinear microscopic imaging could be utilized for the assessment of the skin status of EDS patients in the future.",
keywords = "Collagen structure, Ehlers–Danlos syndrome, Nonlinear microscopy, Second-harmonic generation",
author = "Norbert Kiss and D{\'o}ra Haluszka and Kende Lőrincz and Enikő Kuroli and Judit H{\'a}rsing and B. Mayer and S. K{\'a}rp{\'a}ti and G. Fekete and R. Szip{\'o}cs and N. Wikonk{\'a}l and M{\'a}rta Medvecz",
year = "2018",
month = "5",
day = "3",
doi = "10.1007/s00403-018-1835-7",
language = "English",
pages = "1--11",
journal = "Archiv für Dermatologische Forschung",
issn = "0340-3696",
publisher = "Springer Verlag",

}

TY - JOUR

T1 - Ex vivo nonlinear microscopy imaging of Ehlers–Danlos syndrome-affected skin

AU - Kiss, Norbert

AU - Haluszka, Dóra

AU - Lőrincz, Kende

AU - Kuroli, Enikő

AU - Hársing, Judit

AU - Mayer, B.

AU - Kárpáti, S.

AU - Fekete, G.

AU - Szipócs, R.

AU - Wikonkál, N.

AU - Medvecz, Márta

PY - 2018/5/3

Y1 - 2018/5/3

N2 - Ehlers–Danlos syndrome (EDS) is the name for a heterogenous group of rare genetic connective tissue disorders with an overall incidence of 1 in 5000. The histological characteristics of EDS have been previously described in detail in the late 1970s and early 1980s. Since that time, the classification of EDS has undergone significant changes, yet the description of the histological features of collagen morphology in different EDS subtypes has endured the test of time. Nonlinear microscopy techniques can be utilized for non-invasive in vivo label-free imaging of the skin. Among these techniques, two-photon absorption fluorescence (TPF) microscopy can visualize endogenous fluorophores, such as elastin, while the morphology of collagen fibers can be assessed by second-harmonic generation (SHG) microscopy. In our present work, we performed TPF and SHG microscopy imaging on ex vivo skin samples of one patient with classical EDS and two patients with vascular EDS and two healthy controls. We detected irregular, loosely dispersed collagen fibers in a non-parallel arrangement in the dermis of the EDS patients, while as expected, there was no noticeable impairment in the elastin content. Based on further studies on a larger number of patients, in vivo nonlinear microscopic imaging could be utilized for the assessment of the skin status of EDS patients in the future.

AB - Ehlers–Danlos syndrome (EDS) is the name for a heterogenous group of rare genetic connective tissue disorders with an overall incidence of 1 in 5000. The histological characteristics of EDS have been previously described in detail in the late 1970s and early 1980s. Since that time, the classification of EDS has undergone significant changes, yet the description of the histological features of collagen morphology in different EDS subtypes has endured the test of time. Nonlinear microscopy techniques can be utilized for non-invasive in vivo label-free imaging of the skin. Among these techniques, two-photon absorption fluorescence (TPF) microscopy can visualize endogenous fluorophores, such as elastin, while the morphology of collagen fibers can be assessed by second-harmonic generation (SHG) microscopy. In our present work, we performed TPF and SHG microscopy imaging on ex vivo skin samples of one patient with classical EDS and two patients with vascular EDS and two healthy controls. We detected irregular, loosely dispersed collagen fibers in a non-parallel arrangement in the dermis of the EDS patients, while as expected, there was no noticeable impairment in the elastin content. Based on further studies on a larger number of patients, in vivo nonlinear microscopic imaging could be utilized for the assessment of the skin status of EDS patients in the future.

KW - Collagen structure

KW - Ehlers–Danlos syndrome

KW - Nonlinear microscopy

KW - Second-harmonic generation

UR - http://www.scopus.com/inward/record.url?scp=85046491199&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046491199&partnerID=8YFLogxK

U2 - 10.1007/s00403-018-1835-7

DO - 10.1007/s00403-018-1835-7

M3 - Article

C2 - 29725758

AN - SCOPUS:85046491199

SP - 1

EP - 11

JO - Archiv für Dermatologische Forschung

JF - Archiv für Dermatologische Forschung

SN - 0340-3696

ER -