Ex situ conservation of Dianthus giganteus d'Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers

Liliana Jarda, Anca Butiuc-Keul, Maria Höhn, A. Pedryc, Victoria Cristea

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Ex situ conservation of Dianthus giganteus subsp. banaticus was obtained by in vitro culture on different culture media supplemented with phytohormones. Assessment of somaclonal variability over a period of preservation by in vitro culture is required prior to introduction and acclimatization in natural habitats. In this study we used 2 types of molecular markers, ISSR and multilocus SSR markers, which allow comparison of different alleles, to assess the somaclonal variability of endemic taxa Dianthus giganteus subsp. banaticus after a period of 24 months of preservation by in vitro culture. The assessment of somaclonal variability of D. giganteus subsp. banaticus shows some changes in the absence/presence of certain markers in some individuals.

Original languageEnglish
Pages (from-to)21-30
Number of pages10
JournalTurkish Journal of Biology
Volume38
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

Dianthus
in vitro culture
genetic markers
Plant Growth Regulators
Acclimatization
plant hormones
Ecosystem
Culture Media
acclimation
culture media
Alleles
microsatellite repeats
alleles
habitats
ex situ conservation
tutin
In Vitro Techniques

Keywords

  • Dianthus giganteus subsp. banaticus
  • Ex situ conservation
  • In vitro culture
  • ISSR
  • Somaclonal variability
  • SSR markers

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Physiology
  • Microbiology
  • Cell Biology
  • Agricultural and Biological Sciences(all)

Cite this

Ex situ conservation of Dianthus giganteus d'Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers. / Jarda, Liliana; Butiuc-Keul, Anca; Höhn, Maria; Pedryc, A.; Cristea, Victoria.

In: Turkish Journal of Biology, Vol. 38, No. 1, 2014, p. 21-30.

Research output: Contribution to journalArticle

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