Evolutionary relationships within Aspergillus section Flavi based on sequences of the intergenic transcribed spacer regions and the 5.8S rRNA gene

K. Rigó, J. Varga, Beáta Tóth, J. Téren, Ákos Mesterházy, Zofia Kozakiewicz

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Sequences of the intergenic transcribed spacer regions and the 5.8S rRNA gene (455 nucleotides) of type strains or representative isolates of 23 species and subspecies either currently assigned to Aspergillus subgenus Circumdati section Flavi or other closely related sections, were analyzed. Parsimony analysis of sequence data indicated that species of Aspergillus section Flavi form distinct clades. The three main clades identified based on sequence data could also be distinguished based on colony color, and their ubiquinone systems. The 'A. flavus' clade includes species characterized with Q-10(H2) as their main ubiquinone, conidial colors in shades of green, and dark sclerotia. The 'A. tamarii' clade involves species with ubiquinone system Q-10(H2), and conidia in shades of olive to brown, while the 'A. alliaceus' clade consists of species with Q-10 ubiquinone system, and conidia in shades of ocher. The synnematous species A. coremiiformis was found to be closely related to species in the 'A. tamarii' clade. A. thomii and A. terricola var. americana were found to be related to the 'A. flavus' clade in spite of producing brownish colonies. Three species, A. nomius, A. avenaceus, and A. leporis were found to form separate lineages not closely related to any of the main clades identified. It is suggested that A. clavatoflavus and A. zonatus be excluded from Aspergillus section Flavi. Phylogenetic analysis of partial 26S rRNA gene sequences (564 nucleotides) supported our findings.

Original languageEnglish
Pages (from-to)9-16
Number of pages8
JournalJournal of General and Applied Microbiology
Volume48
Issue number1
Publication statusPublished - 2002

Fingerprint

Intergenic DNA
Ubiquinone
Aspergillus
rRNA Genes
Fungal Spores
Color
Olea
Sequence Analysis
Nucleotides

Keywords

  • 26S rDNA
  • 5.8S rDNA
  • Aspergillus section Flavi
  • Internal transcribed spacers (ITS)
  • ITS region
  • Phylogenetic analysis

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Evolutionary relationships within Aspergillus section Flavi based on sequences of the intergenic transcribed spacer regions and the 5.8S rRNA gene. / Rigó, K.; Varga, J.; Tóth, Beáta; Téren, J.; Mesterházy, Ákos; Kozakiewicz, Zofia.

In: Journal of General and Applied Microbiology, Vol. 48, No. 1, 2002, p. 9-16.

Research output: Contribution to journalArticle

@article{40d97914afbe46d0922c8c9c0ab8abf5,
title = "Evolutionary relationships within Aspergillus section Flavi based on sequences of the intergenic transcribed spacer regions and the 5.8S rRNA gene",
abstract = "Sequences of the intergenic transcribed spacer regions and the 5.8S rRNA gene (455 nucleotides) of type strains or representative isolates of 23 species and subspecies either currently assigned to Aspergillus subgenus Circumdati section Flavi or other closely related sections, were analyzed. Parsimony analysis of sequence data indicated that species of Aspergillus section Flavi form distinct clades. The three main clades identified based on sequence data could also be distinguished based on colony color, and their ubiquinone systems. The 'A. flavus' clade includes species characterized with Q-10(H2) as their main ubiquinone, conidial colors in shades of green, and dark sclerotia. The 'A. tamarii' clade involves species with ubiquinone system Q-10(H2), and conidia in shades of olive to brown, while the 'A. alliaceus' clade consists of species with Q-10 ubiquinone system, and conidia in shades of ocher. The synnematous species A. coremiiformis was found to be closely related to species in the 'A. tamarii' clade. A. thomii and A. terricola var. americana were found to be related to the 'A. flavus' clade in spite of producing brownish colonies. Three species, A. nomius, A. avenaceus, and A. leporis were found to form separate lineages not closely related to any of the main clades identified. It is suggested that A. clavatoflavus and A. zonatus be excluded from Aspergillus section Flavi. Phylogenetic analysis of partial 26S rRNA gene sequences (564 nucleotides) supported our findings.",
keywords = "26S rDNA, 5.8S rDNA, Aspergillus section Flavi, Internal transcribed spacers (ITS), ITS region, Phylogenetic analysis",
author = "K. Rig{\'o} and J. Varga and Be{\'a}ta T{\'o}th and J. T{\'e}ren and {\'A}kos Mesterh{\'a}zy and Zofia Kozakiewicz",
year = "2002",
language = "English",
volume = "48",
pages = "9--16",
journal = "Journal of General and Applied Microbiology",
issn = "0022-1260",
publisher = "Microbiology Research Foundation",
number = "1",

}

TY - JOUR

T1 - Evolutionary relationships within Aspergillus section Flavi based on sequences of the intergenic transcribed spacer regions and the 5.8S rRNA gene

AU - Rigó, K.

AU - Varga, J.

AU - Tóth, Beáta

AU - Téren, J.

AU - Mesterházy, Ákos

AU - Kozakiewicz, Zofia

PY - 2002

Y1 - 2002

N2 - Sequences of the intergenic transcribed spacer regions and the 5.8S rRNA gene (455 nucleotides) of type strains or representative isolates of 23 species and subspecies either currently assigned to Aspergillus subgenus Circumdati section Flavi or other closely related sections, were analyzed. Parsimony analysis of sequence data indicated that species of Aspergillus section Flavi form distinct clades. The three main clades identified based on sequence data could also be distinguished based on colony color, and their ubiquinone systems. The 'A. flavus' clade includes species characterized with Q-10(H2) as their main ubiquinone, conidial colors in shades of green, and dark sclerotia. The 'A. tamarii' clade involves species with ubiquinone system Q-10(H2), and conidia in shades of olive to brown, while the 'A. alliaceus' clade consists of species with Q-10 ubiquinone system, and conidia in shades of ocher. The synnematous species A. coremiiformis was found to be closely related to species in the 'A. tamarii' clade. A. thomii and A. terricola var. americana were found to be related to the 'A. flavus' clade in spite of producing brownish colonies. Three species, A. nomius, A. avenaceus, and A. leporis were found to form separate lineages not closely related to any of the main clades identified. It is suggested that A. clavatoflavus and A. zonatus be excluded from Aspergillus section Flavi. Phylogenetic analysis of partial 26S rRNA gene sequences (564 nucleotides) supported our findings.

AB - Sequences of the intergenic transcribed spacer regions and the 5.8S rRNA gene (455 nucleotides) of type strains or representative isolates of 23 species and subspecies either currently assigned to Aspergillus subgenus Circumdati section Flavi or other closely related sections, were analyzed. Parsimony analysis of sequence data indicated that species of Aspergillus section Flavi form distinct clades. The three main clades identified based on sequence data could also be distinguished based on colony color, and their ubiquinone systems. The 'A. flavus' clade includes species characterized with Q-10(H2) as their main ubiquinone, conidial colors in shades of green, and dark sclerotia. The 'A. tamarii' clade involves species with ubiquinone system Q-10(H2), and conidia in shades of olive to brown, while the 'A. alliaceus' clade consists of species with Q-10 ubiquinone system, and conidia in shades of ocher. The synnematous species A. coremiiformis was found to be closely related to species in the 'A. tamarii' clade. A. thomii and A. terricola var. americana were found to be related to the 'A. flavus' clade in spite of producing brownish colonies. Three species, A. nomius, A. avenaceus, and A. leporis were found to form separate lineages not closely related to any of the main clades identified. It is suggested that A. clavatoflavus and A. zonatus be excluded from Aspergillus section Flavi. Phylogenetic analysis of partial 26S rRNA gene sequences (564 nucleotides) supported our findings.

KW - 26S rDNA

KW - 5.8S rDNA

KW - Aspergillus section Flavi

KW - Internal transcribed spacers (ITS)

KW - ITS region

KW - Phylogenetic analysis

UR - http://www.scopus.com/inward/record.url?scp=0036330569&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036330569&partnerID=8YFLogxK

M3 - Article

C2 - 12469311

AN - SCOPUS:0036330569

VL - 48

SP - 9

EP - 16

JO - Journal of General and Applied Microbiology

JF - Journal of General and Applied Microbiology

SN - 0022-1260

IS - 1

ER -