Evolutionary relationships within Aspergillus section Flavi based on sequences of the intergenic transcribed spacer regions and the 5.8S rRNA gene

Krisztina Rigó, János Varga, Beáta Tóth, József Téren, Ákos Mesterházy, Zofia Kozakiewicz

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Sequences of the intergenic transcribed spacer regions and the 5.8S rRNA gene (455 nucleotides) of type strains or representative isolates of 23 species and subspecies either currently assigned to Aspergillus subgenus Circumdati section Flavi or other closely related sections, were analyzed. Parsimony analysis of sequence data indicated that species of Aspergillus section Flavi form distinct clades. The three main clades identified based on sequence data could also be distinguished based on colony color, and their ubiquinone systems. The 'A. flavus' clade includes species characterized with Q-10(H2) as their main ubiquinone, conidial colors in shades of green, and dark sclerotia. The 'A. tamarii' clade involves species with ubiquinone system Q-10(H2), and conidia in shades of olive to brown, while the 'A. alliaceus' clade consists of species with Q-10 ubiquinone system, and conidia in shades of ocher. The synnematous species A. coremiiformis was found to be closely related to species in the 'A. tamarii' clade. A. thomii and A. terricola var. americana were found to be related to the 'A. flavus' clade in spite of producing brownish colonies. Three species, A. nomius, A. avenaceus, and A. leporis were found to form separate lineages not closely related to any of the main clades identified. It is suggested that A. clavatoflavus and A. zonatus be excluded from Aspergillus section Flavi. Phylogenetic analysis of partial 26S rRNA gene sequences (564 nucleotides) supported our findings.

Original languageEnglish
Pages (from-to)9-16
Number of pages8
JournalJournal of General and Applied Microbiology
Volume48
Issue number1
DOIs
Publication statusPublished - Jan 1 2002

Keywords

  • 26S rDNA
  • 5.8S rDNA
  • Aspergillus section Flavi
  • ITS region
  • Internal transcribed spacers (ITS)
  • Phylogenetic analysis

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Evolutionary relationships within Aspergillus section Flavi based on sequences of the intergenic transcribed spacer regions and the 5.8S rRNA gene'. Together they form a unique fingerprint.

  • Cite this