Evolution of neoplastic development in the liver of transgenic mice co- expressing c-myc and transforming growth factor-α

Eric Santoni-Rugiu, P. Nagy, Michael R. Jensen, Valentina M. Factor, Snorri S. Thorgeirsson

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Abstract

We have previously shown that co-expression of c-myc and transforming growth factor (TGF)-α as transgenes in mouse liver results in major enhancement of neoplastic development in this organ as compared with expression of either of these transgenes alone. In this report we describe in detail the progression from liver cell dysplasia to hepatocellular carcinomas (HCCs) occurring in the liver of c-myc/TGF-α and c-myc transgenic mice. Despite morphological similarities in the sequence of events between the two transgenic lines, the dramatic acceleration, extent, and severity of hepatic lesions in c-myc/TGF-α mice clearly demonstrated the synergistic effects of this transgenic combination. Although c-myc/TGF-α and c-myc females displayed longer latency and lower tumor incidence, the pathological changes were the same as those seen in the male mice, including the formation of HCCs, which are absent in TGF-α single-transgenic females. Tumors in single- and double-transgenic mice showed induction of the endogenous c-myc and TGF- α and, most frequently, unchanged or decreased epidermal growth factor receptor, further indicating the collaborative role of c-myc and TGF-α in providing a selective growth advantage to tumor cells independently of the epidermal growth factor receptor levels. To identify possible tumor precursors, we focused particularly on the dysplastic changes preceding and accompanying the appearance of preneoplastic and neoplastic lesions in the double-transgenic mice. Early on, these changes were characterized by the appearance of large dysplastic hepatocytes, mostly pericentrally, expressing high levels of TGF-α and uPA, as well as TGF-β1, particularly in apoptotic cells. After a short period of replication and expansion into the liver parenchyma, as well as penetration into the central veins, these cells underwent apoptotic cell death while preneoplastic and neoplastic lesions were forming. The peritumorous tissues also contained small dysplastic hepatocytes and oval-like cells, similar to those found in the tumors. Transplantation of the transgenic liver tissues harboring only dysplasia with or without vascular lesions onto nude mice was able to yield HCCs composed of small diploid cells, suggesting that initiated cells are generated during the early dysplastic phase and can progress to HCC. It is therefore likely that large dysplastic hepatocytes undergo apoptosis, which may be closely associated with the up-regulation of TGF-β1 and uPA, whereas other cells evolve into the precursor population for HCC. Due to the simultaneous presence of c-myc, TGF-α, and dysplasia in premalignant human liver diseases, our transgenic mouse system appears to be an appropriate model for studying human hepatocarcinogenesis.

Original languageEnglish
Pages (from-to)407-428
Number of pages22
JournalAmerican Journal of Pathology
Volume149
Issue number2
Publication statusPublished - Aug 1996

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Transforming Growth Factors
Transgenic Mice
Liver
Hepatocellular Carcinoma
Hepatocytes
Neoplasms
Transgenes
Epidermal Growth Factor Receptor
Diploidy
Nude Mice
Liver Transplantation
Blood Vessels
Liver Diseases
Veins
Cell Death
Up-Regulation
Apoptosis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Evolution of neoplastic development in the liver of transgenic mice co- expressing c-myc and transforming growth factor-α. / Santoni-Rugiu, Eric; Nagy, P.; Jensen, Michael R.; Factor, Valentina M.; Thorgeirsson, Snorri S.

In: American Journal of Pathology, Vol. 149, No. 2, 08.1996, p. 407-428.

Research output: Contribution to journalArticle

Santoni-Rugiu, Eric ; Nagy, P. ; Jensen, Michael R. ; Factor, Valentina M. ; Thorgeirsson, Snorri S. / Evolution of neoplastic development in the liver of transgenic mice co- expressing c-myc and transforming growth factor-α. In: American Journal of Pathology. 1996 ; Vol. 149, No. 2. pp. 407-428.
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abstract = "We have previously shown that co-expression of c-myc and transforming growth factor (TGF)-α as transgenes in mouse liver results in major enhancement of neoplastic development in this organ as compared with expression of either of these transgenes alone. In this report we describe in detail the progression from liver cell dysplasia to hepatocellular carcinomas (HCCs) occurring in the liver of c-myc/TGF-α and c-myc transgenic mice. Despite morphological similarities in the sequence of events between the two transgenic lines, the dramatic acceleration, extent, and severity of hepatic lesions in c-myc/TGF-α mice clearly demonstrated the synergistic effects of this transgenic combination. Although c-myc/TGF-α and c-myc females displayed longer latency and lower tumor incidence, the pathological changes were the same as those seen in the male mice, including the formation of HCCs, which are absent in TGF-α single-transgenic females. Tumors in single- and double-transgenic mice showed induction of the endogenous c-myc and TGF- α and, most frequently, unchanged or decreased epidermal growth factor receptor, further indicating the collaborative role of c-myc and TGF-α in providing a selective growth advantage to tumor cells independently of the epidermal growth factor receptor levels. To identify possible tumor precursors, we focused particularly on the dysplastic changes preceding and accompanying the appearance of preneoplastic and neoplastic lesions in the double-transgenic mice. Early on, these changes were characterized by the appearance of large dysplastic hepatocytes, mostly pericentrally, expressing high levels of TGF-α and uPA, as well as TGF-β1, particularly in apoptotic cells. After a short period of replication and expansion into the liver parenchyma, as well as penetration into the central veins, these cells underwent apoptotic cell death while preneoplastic and neoplastic lesions were forming. The peritumorous tissues also contained small dysplastic hepatocytes and oval-like cells, similar to those found in the tumors. Transplantation of the transgenic liver tissues harboring only dysplasia with or without vascular lesions onto nude mice was able to yield HCCs composed of small diploid cells, suggesting that initiated cells are generated during the early dysplastic phase and can progress to HCC. It is therefore likely that large dysplastic hepatocytes undergo apoptosis, which may be closely associated with the up-regulation of TGF-β1 and uPA, whereas other cells evolve into the precursor population for HCC. Due to the simultaneous presence of c-myc, TGF-α, and dysplasia in premalignant human liver diseases, our transgenic mouse system appears to be an appropriate model for studying human hepatocarcinogenesis.",
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