Evolution of DS-1-like human G2P[4] rotaviruses assessed by complete genome analyses

Giovanni M. Giammanco, Floriana Bonura, Mark Zeller, Elisabeth Heylen, Marc Van Ranst, Vito Martella, Kristián Bányai, Jelle Matthijnssens, Simona De Grazia

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31 Citations (Scopus)

Abstract

Group A rotaviruses (RVAs) are a leading cause of viral gastroenteritis in children, with G2P[4] RVA being one of the most common human strains worldwide. The complete genome sequences of nine G2P[4] RVA strains, selected from a 26-year archival collection (1985-2011) established in Palermo, Italy, were determined. A strain associated with a peak of G2P[4] RVA activity in 1996 resembled a reassortant strain identified in Kenya in 1982 and differed completely in genomic make up from more recent strains that circulated during 2004-2011. Conversely, the 2004- 2011 G2P[4] RVAs were genetically more similar to contemporary RVA strains circulating globally. Recent G2P[4] strains possessed either single or multiple genome segments (VP1, VP3 and/or NSP4) likely derived from ruminant viruses through intra-genotype reassortment. Amino acid substitutions were selected and maintained over time in the VP7 and VP8* antigenic proteins, allowing the circulation of two contemporary G2P[4] variants to be distinguished. Altogether, these findings suggest that major changes in the genomic composition of recent G2P[4] RVAs occurred in the early 2000s, leading to the appearance of a novel variant of the DS-1-like genotype constellation. Whether the modifications observed in the neutralizing antigens and in the genome composition of modern G2P[4] RVAs may affect the long-term effectiveness of the vaccination programmes remains to be explored.

Original languageEnglish
Pages (from-to)91-109
Number of pages19
JournalJournal of General Virology
Volume95
Issue numberPART 1
DOIs
Publication statusPublished - 2014

ASJC Scopus subject areas

  • Virology

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    Giammanco, G. M., Bonura, F., Zeller, M., Heylen, E., Van Ranst, M., Martella, V., Bányai, K., Matthijnssens, J., & De Grazia, S. (2014). Evolution of DS-1-like human G2P[4] rotaviruses assessed by complete genome analyses. Journal of General Virology, 95(PART 1), 91-109. https://doi.org/10.1099/vir.0.056788-0