Evidence that the contractile response of the guinea-pig ileum to capsaicin is due to release of substance P

L. Barthó, P. Holzer, F. Lembeck, J. Szolcsányi

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Abstract

The possible roles of substance P and opioids in the contractile response of the isolated guinea-pig ileum to the sensory stimulant drug capsaicin were investigated, and the contractions were found to be inhibited by about 60% in preparations desensitized to substance P. Contractions evoked by stimulation of the mesenteric nerves in the presence of the adrenergic blocking drug guanethidine were inhibited by about 75% after the ileum had been rendered insensitive to substance P. Atropine partially inhibited the effect of capsaicin. The atropine-resistant component of the contractile response to capsaicin was inhibited by more than 85% in preparations desensitized to substance P and almost abolished by the substance P antagonist, (D-Pro2,D-Trp7,9)-substance P. The opioid peptide (D-Met2,Pro5)-enkephalinamide inhibited, whereas the opiate antagonist naloxone enhanced the atropine-resistant contractions in response to capsaicin. The results indicate that the contractile response of the guinea-pig ileum to capsaicin and mesenteric nerve stimulation is mediated by release of substance P, presumably from sensory nerve endings in the gut. Substance P appears to act on the smooth muscle both directly and indirectly via cholinergic neurones. It is proposed that opioids modulate the non-cholinergic response to capsaicin by inhibiting the release of substance P.

Original languageEnglish
Pages (from-to)157-167
Number of pages11
JournalJournal of Physiology
VolumeVol. 332
Publication statusPublished - 1982

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Capsaicin
Substance P
Ileum
Guinea Pigs
Atropine
Opioid Analgesics
Opiate Alkaloids
Guanethidine
Cholinergic Neurons
Opioid Peptides
Sensory Receptor Cells
Naloxone
Adrenergic Agents
Smooth Muscle

ASJC Scopus subject areas

  • Physiology

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Evidence that the contractile response of the guinea-pig ileum to capsaicin is due to release of substance P. / Barthó, L.; Holzer, P.; Lembeck, F.; Szolcsányi, J.

In: Journal of Physiology, Vol. Vol. 332, 1982, p. 157-167.

Research output: Contribution to journalArticle

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