Evidence That ORF 1 and 2 Are the Only Virus-Encoded Replicase Genes of Cymbidium Ringspot Tombusvirus

Ágnes Kollár, József Burgyán

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The 33-kDa and its 92-kDa readthrough protein genes (open reading frames (ORFs) 1 and 2) of cymbidium ringspot tombusvirus (CyRSV) were introduced into Nicotiana benthamiana plants. Protoplasts derived from transgenic plants expressing the first two ORFs were inoculated with in vitro transcripts of defective interfering (DI) RNA. The efficient replication of input DI RNA, which is incapable of autonomous replication, confirmed the biological activity of the expressed viral replicase. In addition, in vitro transcripts of a genomic RNA deletion mutant clone encoding only ORFs 1 and 2 were able to replicate on their own and could support the replication of CyRSV DI RNAs in N. benthamiana protoplasts.

Original languageEnglish
Pages (from-to)169-172
Number of pages4
JournalVirology
Volume201
Issue number1
DOIs
Publication statusPublished - May 15 1994

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Tombusvirus
Open Reading Frames
RNA
Viruses
Protoplasts
Genes
Genetically Modified Plants
DNA Replication
Tobacco
Clone Cells
Proteins

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Evidence That ORF 1 and 2 Are the Only Virus-Encoded Replicase Genes of Cymbidium Ringspot Tombusvirus. / Kollár, Ágnes; Burgyán, József.

In: Virology, Vol. 201, No. 1, 15.05.1994, p. 169-172.

Research output: Contribution to journalArticle

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