Evidence that locustatachykinin I is involved in release of adipokinetic hormone from locust corpora cardiaca

Dick R. Nässel, Paul C C M Passier, K. Elekes, Heinrich Dircksen, Henk G B Vullings, Rafael Cantera

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

The glandular cells of the corpus cardiacum of the locust Locusta migratoria, known to synthesize and release adipokinetic hormones (AKH), are contacted by axons immunoreactive to an antiserum raised against the locust neuropeptide locustatachykinin I (LomTK I). Electron-microscopical immunocytochemistry reveals LomTK immunoreactive axon terminals, containing granular vesicles, in close contact with the glandular cells cells. Release of AKH I from isolated corpora cardiaca of the locust has been monitored in an in vitro system where the amount of AKH I released into the incubation saline is determined by reversed phase high performance liquid chromatography with fluorometric detection. We could show that LomTK I induces release of AKH from corpora cardiaca in a dose-dependent manner when tested in a range of 10-200 μM. This is thus the first clear demonstration of a substance inducing release of AKH, correlated with the presence of the substance in fibers innervating the AKH-synthesizing glandular cells, in the insect corpora cardiaca.

Original languageEnglish
Pages (from-to)297-310
Number of pages14
JournalRegulatory Peptides
Volume57
Issue number3
DOIs
Publication statusPublished - Jun 27 1995

Fingerprint

Corpora Allata
Grasshoppers
Locusta migratoria
Presynaptic Terminals
High performance liquid chromatography
Reverse-Phase Chromatography
Neuropeptides
Axons
Insects
adipokinetic hormone
adipokinetic hormone (locust)
Immune Sera
Demonstrations
Immunohistochemistry
High Pressure Liquid Chromatography
Electrons
Fibers

Keywords

  • Adipokinetic hormone
  • Corpus cardiacum
  • Hormone release
  • Locusta migratoria
  • Locustatachykinin
  • Neuropeptide

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience
  • Neuroscience(all)

Cite this

Evidence that locustatachykinin I is involved in release of adipokinetic hormone from locust corpora cardiaca. / Nässel, Dick R.; Passier, Paul C C M; Elekes, K.; Dircksen, Heinrich; Vullings, Henk G B; Cantera, Rafael.

In: Regulatory Peptides, Vol. 57, No. 3, 27.06.1995, p. 297-310.

Research output: Contribution to journalArticle

Nässel, Dick R. ; Passier, Paul C C M ; Elekes, K. ; Dircksen, Heinrich ; Vullings, Henk G B ; Cantera, Rafael. / Evidence that locustatachykinin I is involved in release of adipokinetic hormone from locust corpora cardiaca. In: Regulatory Peptides. 1995 ; Vol. 57, No. 3. pp. 297-310.
@article{c742f62a8d8a4151af7b09451a1e3196,
title = "Evidence that locustatachykinin I is involved in release of adipokinetic hormone from locust corpora cardiaca",
abstract = "The glandular cells of the corpus cardiacum of the locust Locusta migratoria, known to synthesize and release adipokinetic hormones (AKH), are contacted by axons immunoreactive to an antiserum raised against the locust neuropeptide locustatachykinin I (LomTK I). Electron-microscopical immunocytochemistry reveals LomTK immunoreactive axon terminals, containing granular vesicles, in close contact with the glandular cells cells. Release of AKH I from isolated corpora cardiaca of the locust has been monitored in an in vitro system where the amount of AKH I released into the incubation saline is determined by reversed phase high performance liquid chromatography with fluorometric detection. We could show that LomTK I induces release of AKH from corpora cardiaca in a dose-dependent manner when tested in a range of 10-200 μM. This is thus the first clear demonstration of a substance inducing release of AKH, correlated with the presence of the substance in fibers innervating the AKH-synthesizing glandular cells, in the insect corpora cardiaca.",
keywords = "Adipokinetic hormone, Corpus cardiacum, Hormone release, Locusta migratoria, Locustatachykinin, Neuropeptide",
author = "N{\"a}ssel, {Dick R.} and Passier, {Paul C C M} and K. Elekes and Heinrich Dircksen and Vullings, {Henk G B} and Rafael Cantera",
year = "1995",
month = "6",
day = "27",
doi = "10.1016/0167-0115(95)00043-B",
language = "English",
volume = "57",
pages = "297--310",
journal = "Regulatory Peptides",
issn = "0167-0115",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Evidence that locustatachykinin I is involved in release of adipokinetic hormone from locust corpora cardiaca

AU - Nässel, Dick R.

AU - Passier, Paul C C M

AU - Elekes, K.

AU - Dircksen, Heinrich

AU - Vullings, Henk G B

AU - Cantera, Rafael

PY - 1995/6/27

Y1 - 1995/6/27

N2 - The glandular cells of the corpus cardiacum of the locust Locusta migratoria, known to synthesize and release adipokinetic hormones (AKH), are contacted by axons immunoreactive to an antiserum raised against the locust neuropeptide locustatachykinin I (LomTK I). Electron-microscopical immunocytochemistry reveals LomTK immunoreactive axon terminals, containing granular vesicles, in close contact with the glandular cells cells. Release of AKH I from isolated corpora cardiaca of the locust has been monitored in an in vitro system where the amount of AKH I released into the incubation saline is determined by reversed phase high performance liquid chromatography with fluorometric detection. We could show that LomTK I induces release of AKH from corpora cardiaca in a dose-dependent manner when tested in a range of 10-200 μM. This is thus the first clear demonstration of a substance inducing release of AKH, correlated with the presence of the substance in fibers innervating the AKH-synthesizing glandular cells, in the insect corpora cardiaca.

AB - The glandular cells of the corpus cardiacum of the locust Locusta migratoria, known to synthesize and release adipokinetic hormones (AKH), are contacted by axons immunoreactive to an antiserum raised against the locust neuropeptide locustatachykinin I (LomTK I). Electron-microscopical immunocytochemistry reveals LomTK immunoreactive axon terminals, containing granular vesicles, in close contact with the glandular cells cells. Release of AKH I from isolated corpora cardiaca of the locust has been monitored in an in vitro system where the amount of AKH I released into the incubation saline is determined by reversed phase high performance liquid chromatography with fluorometric detection. We could show that LomTK I induces release of AKH from corpora cardiaca in a dose-dependent manner when tested in a range of 10-200 μM. This is thus the first clear demonstration of a substance inducing release of AKH, correlated with the presence of the substance in fibers innervating the AKH-synthesizing glandular cells, in the insect corpora cardiaca.

KW - Adipokinetic hormone

KW - Corpus cardiacum

KW - Hormone release

KW - Locusta migratoria

KW - Locustatachykinin

KW - Neuropeptide

UR - http://www.scopus.com/inward/record.url?scp=0029069739&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029069739&partnerID=8YFLogxK

U2 - 10.1016/0167-0115(95)00043-B

DO - 10.1016/0167-0115(95)00043-B

M3 - Article

C2 - 7480879

AN - SCOPUS:0029069739

VL - 57

SP - 297

EP - 310

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 0167-0115

IS - 3

ER -