Evidence that IgE molecules mediate a spectrum of effects on mast cell survival and activation via aggregation of the FcεRI

Jiro Kitaura, Jinming Song, Mindy Tsai, Koichi Asai, Mari Maeda-Yamamoto, Attila Mocsai, Yuko Kawakami, Fu Tong Liu, Clifford A. Lowell, B. George Barisas, Stephen J. Galli, Toshiaki Kawakami

Research output: Contribution to journalArticle

212 Citations (Scopus)

Abstract

We demonstrate that binding of different IgE molecules (IgEs) to their receptor, FcεRI, induces a spectrum of activation events in the absence of a specific antigen and provide evidence that such activation reflects aggregation of FcεRI. Highly cytokinergic IgEs can efficiently induce production of cytokines and render mast cells resistant to apoptosis in an autocrine fashion, whereas poorly cytokinergic IgEs induce these effects inefficiently. Highly cytokinergic IgEs seem to induce more extensive FcεRI aggregation than do poorly cytokinergic IgEs, which leads to stronger mast cell activation and survival effects. These effects of both types of IgEs require Syk tyrosine kinase and can be inhibited by FcεRI disaggregation with monovalent hapten. In hybridoma-transplanted mice, mucosal mast cell numbers correlate with serum IgE levels. Therefore, survival effects of IgE could contribute to the pathogenesis of allergic disease.

Original languageEnglish
Pages (from-to)12911-12916
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number22
DOIs
Publication statusPublished - Oct 28 2003

Fingerprint

Mast Cells
Immunoglobulin E
Cell Survival
Haptens
Hybridomas
Protein-Tyrosine Kinases
Cell Count
Apoptosis
Cytokines
Antigens
Serum

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Evidence that IgE molecules mediate a spectrum of effects on mast cell survival and activation via aggregation of the FcεRI. / Kitaura, Jiro; Song, Jinming; Tsai, Mindy; Asai, Koichi; Maeda-Yamamoto, Mari; Mocsai, Attila; Kawakami, Yuko; Liu, Fu Tong; Lowell, Clifford A.; Barisas, B. George; Galli, Stephen J.; Kawakami, Toshiaki.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 22, 28.10.2003, p. 12911-12916.

Research output: Contribution to journalArticle

Kitaura, J, Song, J, Tsai, M, Asai, K, Maeda-Yamamoto, M, Mocsai, A, Kawakami, Y, Liu, FT, Lowell, CA, Barisas, BG, Galli, SJ & Kawakami, T 2003, 'Evidence that IgE molecules mediate a spectrum of effects on mast cell survival and activation via aggregation of the FcεRI', Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 22, pp. 12911-12916. https://doi.org/10.1073/pnas.1735525100
Kitaura, Jiro ; Song, Jinming ; Tsai, Mindy ; Asai, Koichi ; Maeda-Yamamoto, Mari ; Mocsai, Attila ; Kawakami, Yuko ; Liu, Fu Tong ; Lowell, Clifford A. ; Barisas, B. George ; Galli, Stephen J. ; Kawakami, Toshiaki. / Evidence that IgE molecules mediate a spectrum of effects on mast cell survival and activation via aggregation of the FcεRI. In: Proceedings of the National Academy of Sciences of the United States of America. 2003 ; Vol. 100, No. 22. pp. 12911-12916.
@article{36ddb4990e6b4d0298244a31240ab545,
title = "Evidence that IgE molecules mediate a spectrum of effects on mast cell survival and activation via aggregation of the FcεRI",
abstract = "We demonstrate that binding of different IgE molecules (IgEs) to their receptor, FcεRI, induces a spectrum of activation events in the absence of a specific antigen and provide evidence that such activation reflects aggregation of FcεRI. Highly cytokinergic IgEs can efficiently induce production of cytokines and render mast cells resistant to apoptosis in an autocrine fashion, whereas poorly cytokinergic IgEs induce these effects inefficiently. Highly cytokinergic IgEs seem to induce more extensive FcεRI aggregation than do poorly cytokinergic IgEs, which leads to stronger mast cell activation and survival effects. These effects of both types of IgEs require Syk tyrosine kinase and can be inhibited by FcεRI disaggregation with monovalent hapten. In hybridoma-transplanted mice, mucosal mast cell numbers correlate with serum IgE levels. Therefore, survival effects of IgE could contribute to the pathogenesis of allergic disease.",
author = "Jiro Kitaura and Jinming Song and Mindy Tsai and Koichi Asai and Mari Maeda-Yamamoto and Attila Mocsai and Yuko Kawakami and Liu, {Fu Tong} and Lowell, {Clifford A.} and Barisas, {B. George} and Galli, {Stephen J.} and Toshiaki Kawakami",
year = "2003",
month = "10",
day = "28",
doi = "10.1073/pnas.1735525100",
language = "English",
volume = "100",
pages = "12911--12916",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "22",

}

TY - JOUR

T1 - Evidence that IgE molecules mediate a spectrum of effects on mast cell survival and activation via aggregation of the FcεRI

AU - Kitaura, Jiro

AU - Song, Jinming

AU - Tsai, Mindy

AU - Asai, Koichi

AU - Maeda-Yamamoto, Mari

AU - Mocsai, Attila

AU - Kawakami, Yuko

AU - Liu, Fu Tong

AU - Lowell, Clifford A.

AU - Barisas, B. George

AU - Galli, Stephen J.

AU - Kawakami, Toshiaki

PY - 2003/10/28

Y1 - 2003/10/28

N2 - We demonstrate that binding of different IgE molecules (IgEs) to their receptor, FcεRI, induces a spectrum of activation events in the absence of a specific antigen and provide evidence that such activation reflects aggregation of FcεRI. Highly cytokinergic IgEs can efficiently induce production of cytokines and render mast cells resistant to apoptosis in an autocrine fashion, whereas poorly cytokinergic IgEs induce these effects inefficiently. Highly cytokinergic IgEs seem to induce more extensive FcεRI aggregation than do poorly cytokinergic IgEs, which leads to stronger mast cell activation and survival effects. These effects of both types of IgEs require Syk tyrosine kinase and can be inhibited by FcεRI disaggregation with monovalent hapten. In hybridoma-transplanted mice, mucosal mast cell numbers correlate with serum IgE levels. Therefore, survival effects of IgE could contribute to the pathogenesis of allergic disease.

AB - We demonstrate that binding of different IgE molecules (IgEs) to their receptor, FcεRI, induces a spectrum of activation events in the absence of a specific antigen and provide evidence that such activation reflects aggregation of FcεRI. Highly cytokinergic IgEs can efficiently induce production of cytokines and render mast cells resistant to apoptosis in an autocrine fashion, whereas poorly cytokinergic IgEs induce these effects inefficiently. Highly cytokinergic IgEs seem to induce more extensive FcεRI aggregation than do poorly cytokinergic IgEs, which leads to stronger mast cell activation and survival effects. These effects of both types of IgEs require Syk tyrosine kinase and can be inhibited by FcεRI disaggregation with monovalent hapten. In hybridoma-transplanted mice, mucosal mast cell numbers correlate with serum IgE levels. Therefore, survival effects of IgE could contribute to the pathogenesis of allergic disease.

UR - http://www.scopus.com/inward/record.url?scp=0242331616&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0242331616&partnerID=8YFLogxK

U2 - 10.1073/pnas.1735525100

DO - 10.1073/pnas.1735525100

M3 - Article

VL - 100

SP - 12911

EP - 12916

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 22

ER -