Evidence that adrenergic and cholinergic but not capsaicin-sensitive nerves are involved in the nerve-mediated contraction of the guinea pig seminal vesicle

R. Benkó, Veronika Szombati, L. Barthó

Research output: Contribution to journalArticle

Abstract

Background/Aims: The neurotransmitters participating in the nerve-mediated contraction of the guinea pig seminal vesicle (GPSV) have not been firmly established. There is debate as to the mediating role of norepinephrine and acetylcholine. Methods: We have used longitudinally and circularly oriented strips of GPSV and activated their intramural nerves by electrical field stimulation (5 and 10 Hz for 30 s). Results: Contractile responses to stimulation were enhanced by a cholinesterase inhibitor and reduced by the adrenergic α-receptor antagonist phentolamine (2.5 μmol/l). Atropine (1 μmol/l) significantly reduced responses in longitudinal preparations; a less consistent inhibition was found in circular preparations. The sensory neuron stimulant and blocker capsaicin was without effect. Conclusions: It is concluded that adrenergic nerves and also acetylcholine mediate the contractile response of the GPSV.

Original languageEnglish
Pages (from-to)39-42
Number of pages4
JournalPharmacology
Volume92
Issue number1-2
DOIs
Publication statusPublished - Sep 2013

Fingerprint

Seminal Vesicles
Capsaicin
Adrenergic Agents
Cholinergic Agents
Guinea Pigs
Acetylcholine
Adrenergic Antagonists
Phentolamine
Cholinesterase Inhibitors
Sensory Receptor Cells
Atropine
Electric Stimulation
Neurotransmitter Agents
Norepinephrine

Keywords

  • Acetylcholine
  • Capsaicin
  • Guinea pig
  • Noradrenaline
  • Seminal vesicle

ASJC Scopus subject areas

  • Pharmacology

Cite this

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abstract = "Background/Aims: The neurotransmitters participating in the nerve-mediated contraction of the guinea pig seminal vesicle (GPSV) have not been firmly established. There is debate as to the mediating role of norepinephrine and acetylcholine. Methods: We have used longitudinally and circularly oriented strips of GPSV and activated their intramural nerves by electrical field stimulation (5 and 10 Hz for 30 s). Results: Contractile responses to stimulation were enhanced by a cholinesterase inhibitor and reduced by the adrenergic α-receptor antagonist phentolamine (2.5 μmol/l). Atropine (1 μmol/l) significantly reduced responses in longitudinal preparations; a less consistent inhibition was found in circular preparations. The sensory neuron stimulant and blocker capsaicin was without effect. Conclusions: It is concluded that adrenergic nerves and also acetylcholine mediate the contractile response of the GPSV.",
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AU - Benkó, R.

AU - Szombati, Veronika

AU - Barthó, L.

PY - 2013/9

Y1 - 2013/9

N2 - Background/Aims: The neurotransmitters participating in the nerve-mediated contraction of the guinea pig seminal vesicle (GPSV) have not been firmly established. There is debate as to the mediating role of norepinephrine and acetylcholine. Methods: We have used longitudinally and circularly oriented strips of GPSV and activated their intramural nerves by electrical field stimulation (5 and 10 Hz for 30 s). Results: Contractile responses to stimulation were enhanced by a cholinesterase inhibitor and reduced by the adrenergic α-receptor antagonist phentolamine (2.5 μmol/l). Atropine (1 μmol/l) significantly reduced responses in longitudinal preparations; a less consistent inhibition was found in circular preparations. The sensory neuron stimulant and blocker capsaicin was without effect. Conclusions: It is concluded that adrenergic nerves and also acetylcholine mediate the contractile response of the GPSV.

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KW - Acetylcholine

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KW - Noradrenaline

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