Evidence for the transport of glutathione through ryanodine receptor channel type 1

Gábor Bánhegyi, Miklós Csala, Gábor Nagy, Vincenzo Sorrentino, Rosella Fulceri, Angelo Benedetti

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In the present study, we have investigated the role of RyR1 (ryanodine receptor calcium channel type 1) in glutathione (GSH) transport through the sarcoplasmic reticulum (SR) membrane of skeletal muscles. Lanthanum chloride, a prototypic blocker of cation channels, inhibited the influx and efflux of GSH in SR vesicles. Using a rapid-filtration-based assay and lanthanum chloride as a transport blocker, an uptake of radiolabelled GSH into SR vesicles was observed. Pretreatment of SR vesicles with the RyR1 antagonists Ruthenium Red and ryanodine as well as with lanthanum chloride blocked the GSH uptake. An SR-like GSH uptake appeared in microsomes obtained from an HEK-293 (human embryonic kidney 293) cell line after transfection of RyR1. These observations strongly suggest that RyR1 mediates GSH transport through the SR membranes of skeletal muscles.

Original languageEnglish
Pages (from-to)807-812
Number of pages6
JournalBiochemical Journal
Volume376
Issue number3
DOIs
Publication statusPublished - Dec 15 2003

Keywords

  • Cation channel
  • Glutathione
  • Lanthanum chloride
  • Ryanodine receptor calcium channel type 1
  • Sarcoplasmic reticulum

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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