Evidence for the involvement of oxygen free radicals in the ethanol- induced late cellular injury in mouse myeloma cells

Beáta Bódis, Oszkár Karádi, Imre Szabó, P. Németh, J. Belágyi, G. Mózsik

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In this study, we analysed the ethanol-induced long term cell injury on a general cell model (Sp2/0-Ag14 cell line). Cells were incubated in 1, 5, 10, 15 and 20% of ethanol (EtOH) for 5 min. After washing cell viability was tested by the Trypan Blue exclusion test in 5, 60 min, 4 and 24 h after EtOH exposure. Free radicals were monitored every 30 min by electron spin resonance (ESR) with alpha-phenyl-N-tert-butylnitrone (PBN) spin trapping technique. Scavenger compounds such as glutathione (GSH), dimethyl sulfoxide (DMSO) and 5,5-dimethyl-1-pyrroline N-oxide (DMPO) were applied for 24 h incubation after EtOH exposure. EtOH concentration dependently decreased the cell viability immediately after 5 min exposure, but with 4 and 24 h, a secondary cell destruction was found. Using ESR-spin trapping technique, an increased free radical activity could be detected. DMPO, DMSO and GSH significantly, but in different period protected the cells against free- radical induced cellular damage. EtOH produces an early (immediately after EtOH exposure) and a late (in about 4 h) cellular damage on Sp2/0-Ag14 cells. The oxygen free radicals can be detected in a short time after EtOH exposure, its biological effect manifested as a secondary cell destruction at 4 and 24 h. This phenomenon can be prevented by scavenger compounds. (C) 2000 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)67-70
Number of pages4
JournalJournal of Physiology Paris
Volume94
Issue number1
DOIs
Publication statusPublished - Jan 2000

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Free Radicals
Reactive Oxygen Species
Ethanol
Wounds and Injuries
Spin Trapping
Electron Spin Resonance Spectroscopy
Dimethyl Sulfoxide
Cell Survival
Trypan Blue
Oxides
Glutathione
Cell Line

Keywords

  • ESR-spin trapping method
  • Ethanol
  • Oxygen free radicals
  • Sp2/0-Ag14 cell line

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology (medical)

Cite this

Evidence for the involvement of oxygen free radicals in the ethanol- induced late cellular injury in mouse myeloma cells. / Bódis, Beáta; Karádi, Oszkár; Szabó, Imre; Németh, P.; Belágyi, J.; Mózsik, G.

In: Journal of Physiology Paris, Vol. 94, No. 1, 01.2000, p. 67-70.

Research output: Contribution to journalArticle

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