Evidence for the involvement of oxygen free radicals in the ethanol- induced late cellular injury in mouse myeloma cells

Beáta Bódis, Oszkár Karádi, Imre Szabó, Péter Németh, József Belágyi, Gyula Mózsik

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In this study, we analysed the ethanol-induced long term cell injury on a general cell model (Sp2/0-Ag14 cell line). Cells were incubated in 1, 5, 10, 15 and 20% of ethanol (EtOH) for 5 min. After washing cell viability was tested by the Trypan Blue exclusion test in 5, 60 min, 4 and 24 h after EtOH exposure. Free radicals were monitored every 30 min by electron spin resonance (ESR) with alpha-phenyl-N-tert-butylnitrone (PBN) spin trapping technique. Scavenger compounds such as glutathione (GSH), dimethyl sulfoxide (DMSO) and 5,5-dimethyl-1-pyrroline N-oxide (DMPO) were applied for 24 h incubation after EtOH exposure. EtOH concentration dependently decreased the cell viability immediately after 5 min exposure, but with 4 and 24 h, a secondary cell destruction was found. Using ESR-spin trapping technique, an increased free radical activity could be detected. DMPO, DMSO and GSH significantly, but in different period protected the cells against free- radical induced cellular damage. EtOH produces an early (immediately after EtOH exposure) and a late (in about 4 h) cellular damage on Sp2/0-Ag14 cells. The oxygen free radicals can be detected in a short time after EtOH exposure, its biological effect manifested as a secondary cell destruction at 4 and 24 h. This phenomenon can be prevented by scavenger compounds. (C) 2000 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)67-70
Number of pages4
JournalJournal of Physiology Paris
Volume94
Issue number1
DOIs
Publication statusPublished - Jan 2000

Keywords

  • ESR-spin trapping method
  • Ethanol
  • Oxygen free radicals
  • Sp2/0-Ag14 cell line

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Evidence for the involvement of oxygen free radicals in the ethanol- induced late cellular injury in mouse myeloma cells'. Together they form a unique fingerprint.

  • Cite this