Evidence for Orientation-Conserved Transfer in the TCA Cycle in Saccharomyces cerevisiae: 13C NMR Studies

B. Sumegi, A. D. Sherry, C. R. Malloy, P. A. Srere

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Abstract

When [4-13C]glutamate is incubated with yeast cells, the [13C]aspartate formed shows a [2-13C]/[3-13C] ratio that is greater than 1. If the conversion occurs through the Krebs TCA cycle with the symmetrical intermediates succinate and fumarate, the ratio is expected to be 1. The addition of fluoroacetate to the incubation increases the ratio further. When the mutants Aco1 or CS1 are used, little or no aspartate is formed. When [2-13C]acetate is incubated with yeast, the C2-/C3-aspartate ratio is 1.5 at 2 min and decreases to 1.0 after 10 min of incubation. These results indicate that orientation-conserved transfer occurs at the succinate thiokinase and succinate dehydrogenase steps of the Krebs TCA cycle.

Original languageEnglish
Pages (from-to)12725-12729
Number of pages5
JournalBiochemistry
Volume32
Issue number47
DOIs
Publication statusPublished - Jan 1 1993

ASJC Scopus subject areas

  • Biochemistry

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